Correa-Fiz Florencia, Galofre-Mila Nuria, Costa-Hurtado Mar, Aragon Virginia
IRTA, Centre de Recerca en Sanitat Animal (CReSA, IRTA-UAB), Campus de la Universitat Autònoma de Barcelona, 08193, Bellaterra, Spain.
IRTA, Centre de Recerca en Sanitat Animal (CReSA, IRTA-UAB), Campus de la Universitat Autònoma de Barcelona, 08193, Bellaterra, Spain.
Vet Microbiol. 2017 Jan;198:116-120. doi: 10.1016/j.vetmic.2016.12.015. Epub 2016 Dec 16.
Haemophilus parasuis is a bacterium from the Pasteurellaceae family that comprises strains of different degree of virulence. Non-virulent strains are considered components of the upper respiratory tract microbiota, while virulent strains can invade systemic organs and cause fibrinous polyserositis (Glässer's disease). Genomic comparison of virulent and non-virulent strains led to the identification of a family of genes differentially associated to virulence, the virulence-associated trimeric autotransporters (vtaA). Monoclonal antibody 69C6 reacted with the surface of virulent strains and has allowed now the identification of an epitope in the C terminus of the passenger domain of the VtaAs from virulent strains. Protein modelling indicated that the epitope is probably exposed, although sera from pigs vaccinated with the passenger domain of VtaA9 and from convalescent animals did not react with the 69C6 epitope. Induction of antibodies against the 69C6 epitope by vaccination would allow a response targeting specifically virulent strains of H. parasuis.
副猪嗜血杆菌是巴氏杆菌科的一种细菌，它包含不同毒力程度的菌株。无毒力菌株被认为是上呼吸道微生物群的组成部分，而有毒力菌株可侵入全身器官并引起纤维素性多浆膜炎（格拉泽氏病）。对有毒力和无毒力菌株的基因组比较导致鉴定出一类与毒力差异相关的基因，即毒力相关三聚体自转运蛋白（vtaA）。单克隆抗体69C6与有毒力菌株的表面发生反应，现在已能够鉴定出有毒力菌株VtaAs乘客结构域C末端的一个表位。蛋白质建模表明该表位可能是暴露的，尽管用VtaA9乘客结构域免疫接种的猪和恢复期动物的血清未与69C6表位发生反应。通过疫苗接种诱导针对69C6表位的抗体将产生针对副猪嗜血杆菌有毒力菌株的特异性反应。
