Sano T, Nagayasu S, Suzuki S, Iwashita M, Yamashita A, Shinjo T, Sanui T, Kushiyama A, Kanematsu T, Asano T, Nishimura F
Section of Periodontology, Kyushu University Faculty of Dental Science, Fukuoka, Japan.
Department of Dental Science for Health Promotion, Hiroshima University Institute of Biomedical and Health Sciences, Hiroshima, Japan.
Nutr Metab Cardiovasc Dis. 2017 Mar;27(3):249-259. doi: 10.1016/j.numecd.2016.11.008. Epub 2016 Nov 23.
Epicatechin (EC) intake has been suggested to be beneficial for the prevention of cardiovascular disorders, and it is well known that adipose tissue inflammation is one of the major risk factors for coronary heart diseases. The purpose of the present study was to determine the in vitro and in vivo effects of EC on adipose tissue inflammation and obesity.
DNA microarray analysis was performed to evaluate the effects of EC on gene expression in adipocytes co-cultured with bacterial endotoxin-stimulated macrophages. To determine the in vivo effects of the catechin, C57BL/6 mice were fed either a high-fat diet (HFD) or HFD combined with EC, and metabolic changes were observed EC suppressed the expression of many inflammatory genes in the adipocytes co-cultured with endotoxin-stimulated macrophages. Specifically, EC markedly suppressed chemokine (CC motif) ligand 19 (CCL19) expression. The target cell of EC appeared to macrophages. The in vivo study indicated that mice fed the EC-supplemented HFD were protected from diet-induced obesity and insulin resistance. Accordingly, the expression levels of genes associated with inflammation in adipose tissue and in the liver were downregulated in this group of mice.
EC exerts beneficial effects for the prevention of adipose tissue inflammation and insulin resistance. Since we previously reported that mice deficient in the CCL19 receptor were protected from diet-induced obesity and insulin resistance, it can be concluded that the beneficial effects of EC could be mediated, at least in part, by marked suppression of CCL19 expression.
摄入表儿茶素（EC）被认为对预防心血管疾病有益，众所周知，脂肪组织炎症是冠心病的主要危险因素之一。本研究的目的是确定EC在体外和体内对脂肪组织炎症和肥胖的影响。
进行DNA微阵列分析以评估EC对与细菌内毒素刺激的巨噬细胞共培养的脂肪细胞中基因表达的影响。为了确定儿茶素的体内作用，给C57BL/6小鼠喂食高脂饮食（HFD）或HFD与EC的组合，并观察代谢变化。EC抑制了与内毒素刺激的巨噬细胞共培养的脂肪细胞中许多炎症基因的表达。具体而言，EC显著抑制趋化因子（CC基序）配体19（CCL19）的表达。EC的靶细胞似乎是巨噬细胞。体内研究表明，喂食补充EC的HFD的小鼠可免受饮食诱导的肥胖和胰岛素抵抗。因此，该组小鼠脂肪组织和肝脏中与炎症相关的基因表达水平下调。
EC对预防脂肪组织炎症和胰岛素抵抗具有有益作用。由于我们之前报道缺乏CCL19受体的小鼠可免受饮食诱导的肥胖和胰岛素抵抗，因此可以得出结论，EC的有益作用至少部分可通过显著抑制CCL19表达来介导。
