Ma Chao, Wang Jianqi, Fan Longkun, Guo Yanjun
Department of oral and maxillofacial surgery, Cangzhou central hospital of Hebei province, Cangzhou 061001, China.
Department of oral and maxillofacial surgery, Cangzhou central hospital of Hebei province, Cangzhou 061001, China.
Exp Mol Pathol. 2017 Feb;102(1):59-64. doi: 10.1016/j.yexmp.2017.01.002. Epub 2017 Jan 4.
Head and neck squamous cell carcinoma (HNSCC) is one of the most common cancers in the world. CD147, a transmembrane glycoprotein, has been reported to be correlated with cancer progression, metastasis, and chemoresistance in various cancers. In this study, we aimed to investigate the mechanism of CD147 in regulating drug resistance in HNSCC cells. qRT-PCR were used to evaluated the expression of CD147 in 57 HNSCC tumorous tissues and 2 cell lines. Increased expression of CD147 was found in most HNSCC samples, and the expression level of CD147 was correlated with multidrug resistance. CD147 RNA silencing decreased the chemoresistance of HNSCC cells by deactivating MAPK/ERK signaling pathway. Further investigation revealed that either rescue expression of CD147 or treatment of MAPK/ERK activator phorbol 12-myristate 13-acetate (PMA) in CD147 knockdown CRC cell line attenuated the decreased chemoresistance in CD147 knockdown cells. Taken together, our results suggest that CD147 promotes chemoresistance by activating MAPK/ERK signaling pathway in HNSCC.
头颈部鳞状细胞癌（HNSCC）是世界上最常见的癌症之一。CD147是一种跨膜糖蛋白，据报道在各种癌症中与癌症进展、转移和化疗耐药性相关。在本研究中，我们旨在探讨CD147在调节HNSCC细胞耐药性中的机制。采用qRT-PCR检测57例HNSCC肿瘤组织和2种细胞系中CD147的表达。在大多数HNSCC样本中发现CD147表达增加，且CD147表达水平与多药耐药性相关。CD147 RNA沉默通过失活MAPK/ERK信号通路降低了HNSCC细胞的化疗耐药性。进一步研究发现，在CD147敲低的CRC细胞系中，CD147的拯救表达或MAPK/ERK激活剂佛波醇12-肉豆蔻酸酯13-乙酸酯（PMA）处理均可减弱CD147敲低细胞中化疗耐药性的降低。综上所述，我们的结果表明CD147通过激活HNSCC中的MAPK/ERK信号通路促进化疗耐药性。
