Valadbeigi Hassan, Sadeghifard Nourkhoda, Salehi Majid Baseri
Department of Microbiology, Science and Research Branch, Islamic Azad University, Fars, Iran; Department of Microbiology, Shiraz Branch, Islamic Azad University, Shiraz, Iran.
Clinical Microbiology Research Center, Ilam University of Medical Sciences, Ilam, Iran.
Microb Pathog. 2017 Mar;104:28-31. doi: 10.1016/j.micpath.2017.01.009. Epub 2017 Jan 3.
The hallmark patogenicity in Pseudomonas aeruginosa (P. aeruginosa) is biofilm formation that is not easy to eradicate, because it has variety mechanisms for antibiotic resistance. In addition, toxin-antitoxin (TA) system may play role in biofilm formation. The current study aimed to evaluate the role of TA loci in biofilm formation. Therefore, 18 P. aeruginosa clinical isolates were collected and evaluated for specific biofilm and TA genes. The analysis by RT-qPCR demonstrated that expression of mazE antitoxin in biofilm formation was increase. On the other hand, mazE antitoxin TA system was used as target for antisense PNA. mazE-PNA was able to influence in biofilm formation and was inhibit at 5,10 and 15 μM concentrations biofilm formation in P. aeruginosa. Therefore, it could be highlighted target for anti-biofilm target to eradicate P. aeruginosa biofilm producer.
铜绿假单胞菌（P. aeruginosa）的标志性致病性在于生物膜形成，这种生物膜不易根除，因为它具有多种抗生素耐药机制。此外，毒素 - 抗毒素（TA）系统可能在生物膜形成中发挥作用。当前研究旨在评估TA基因座在生物膜形成中的作用。因此，收集了18株铜绿假单胞菌临床分离株，并对特定的生物膜和TA基因进行评估。RT - qPCR分析表明，mazE抗毒素在生物膜形成中的表达增加。另一方面，mazE抗毒素TA系统被用作反义肽核酸（PNA）的靶点。mazE - PNA能够影响生物膜形成，并在5、10和15μM浓度下抑制铜绿假单胞菌的生物膜形成。因此，它可能是根除产生物膜铜绿假单胞菌的抗生物膜靶点中的突出靶点。
