膜联蛋白A1在易患动脉粥样硬化小鼠动脉内膜形成中的保护作用——简要报告
Protective Aptitude of Annexin A1 in Arterial Neointima Formation in Atherosclerosis-Prone Mice-Brief Report.
作者信息
de Jong Renske J, Paulin Nicole, Lemnitzer Patricia, Viola Joana R, Winter Carla, Ferraro Bartolo, Grommes Jochen, Weber Christian, Reutelingsperger Chris, Drechsler Maik, Soehnlein Oliver
机构信息
From the IPEK, LMU Munich, Germany (R.J.d.J., N.P., P.L., J.R.V., C. Winter, B.F., J.G., C. Weber, M.D., O.S.); Department of Pathology, AMC, Amsterdam University, The Netherlands (R.J.d.J., J.R.V., M.D., O.S.); Department of Experimental Medicine, Second University of Naples, Italy (B.F.); European Vascular Center Aachen-Maastricht, University Hospital RWTH Aachen, Germany (J.G.); Department of Biochemistry, CARIM, Maastricht University, The Netherlands (C. Weber, C.R.); and DZHK, partner site Munich Heart Alliance, Germany (C. Weber, M.D., O.S.).
出版信息
Arterioscler Thromb Vasc Biol. 2017 Feb;37(2):312-315. doi: 10.1161/ATVBAHA.116.308744. Epub 2016 Dec 29.
OBJECTIVE
Restenosis as a consequence of arterial injury is aggravated by inflammatory pathways. Here, we investigate the role of the proresolving protein annexin A1 (AnxA1) in healing after wire injury.
APPROACH AND RESULTS
Apoe and ApoeAnxa1 mice were subjected to wire injury while fed a high-cholesterol diet. Subsequently, localization of AnxA1 and AnxA1 plasma levels were examined. AnxA1 was found to localize within endothelial cells and macrophages in the neointima. Levels of AnxA1 in the plasma and its lesional expression negatively correlated with neointima size, and in the absence of AnxA1, neointima formation was aggravated by the accumulation and proliferation of macrophages. In contrast, reendothelialization and smooth muscle cell infiltration were not affected in ApoeAnxa1 mice.
CONCLUSIONS
AnxA1 is protective in healing after wire injury and could, therefore, be an attractive therapeutic compound to prevent from restenosis after vascular damage.
目的
动脉损伤导致的再狭窄会因炎症途径而加重。在此，我们研究促消退蛋白膜联蛋白A1（AnxA1）在钢丝损伤后愈合过程中的作用。
方法与结果
给载脂蛋白E基因敲除（Apoe）小鼠和载脂蛋白E基因敲除/膜联蛋白A1基因敲除（ApoeAnxa1）小鼠喂食高胆固醇饮食，同时对其进行钢丝损伤。随后，检测AnxA1的定位及血浆水平。发现AnxA1定位于新生内膜中的内皮细胞和巨噬细胞内。血浆中AnxA1水平及其在损伤部位的表达与新生内膜大小呈负相关，且在缺乏AnxA1的情况下，巨噬细胞的积聚和增殖会加重新生内膜的形成。相比之下，ApoeAnxa1小鼠的再内皮化和平滑肌细胞浸润未受影响。
结论
AnxA1对钢丝损伤后的愈合具有保护作用，因此可能是一种有吸引力的治疗化合物，可预防血管损伤后的再狭窄。
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