School of Life Sciences, Sambalpur University, Jyotivihar, Burla, Sambalpur, Odisha, 768019, India.
Institute of Science, Nirma University, Sarkhej-Gandhinagar Highway, Ahmedabad, Gujarat, 382481, India.
Probiotics Antimicrob Proteins. 2017 Sep;9(3):345-354. doi: 10.1007/s12602-016-9249-3.
Bacteriocin TSU4 is a novel antimicrobial peptide isolated from Catla catla gut isolate Lactobacillus animalis TSU4. It has been reported for its potential antimicrobial activity against fish pathogens and food spoilage bacteria. In vivo safety evaluation is necessary to determine its immunogenicity, toxicity, and importance in real-life applications. The present study was designed to evaluate the immunogenicity, acute and sub-chronic toxicity of bacteriocin TSU4 in BALB/c mice to ensure its safety in industrial application. Male BALB/c mice were administered intraperitoneally for immunogenicity assessment, by oral gavage with 50, 100, and 200 mg/kg/body weight for acute test and 0.5 mg/kg/day dose of bacteriocin TSU4 for sub-chronic toxicity test. Neither mortality nor any infections were observed during experimental period. There was no major increase in antibody titer during the immunogenicity test, and no mortality was observed during acute or sub-chronic toxicity tests. The LD value of bacteriocin TSU4 was found to be higher than 200 ± 0.45 mg/kg. No significant change in the serum biochemical markers, histopathological analysis and visual observation in spleen sizes was observed. These findings revealed that bacteriocin TSU4 is a non-immunogenic, safe, non-toxic, and could be a potential candidate for industrial applications in food preservation and aquaculture industries.
细菌素 TSU4 是一种从鲶鱼肠道分离的乳杆菌中分离出来的新型抗菌肽。它已被报道具有潜在的抗鱼类病原体和食源腐败菌的抗菌活性。为了确定其免疫原性、毒性和在实际应用中的重要性，有必要进行体内安全性评估。本研究旨在评估细菌素 TSU4 在 BALB/c 小鼠中的免疫原性、急性和亚慢性毒性，以确保其在工业应用中的安全性。雄性 BALB/c 小鼠进行了免疫原性评估，通过腹腔内注射和口服灌胃 50、100 和 200mg/kg/体重进行急性试验，以及每天 0.5mg/kg 的细菌素 TSU4 剂量进行亚慢性毒性试验。在实验期间，未观察到死亡或任何感染。在免疫原性试验中，抗体滴度没有明显增加，在急性或亚慢性毒性试验中也没有观察到死亡。细菌素 TSU4 的 LD 值大于 200±0.45mg/kg。血清生化标志物、组织病理学分析和脾脏大小的肉眼观察均无明显变化。这些发现表明，细菌素 TSU4 是一种非免疫原性、安全、无毒的物质，可能是食品保鲜和水产养殖行业工业应用的潜在候选物。
