Venault Antoine, Hsu Ko-Jen, Yeh Lu-Chen, Chinnathambi Arunachalam, Ho Hsin-Tsung, Chang Yung
R&D Center for Membrane Technology and Department of Chemical Engineering, Chung Yuan Christian University, Chung-Li, Taoyuan 320, Taiwan.
R&D Center for Membrane Technology and Department of Chemical Engineering, Chung Yuan Christian University, Chung-Li, Taoyuan 320, Taiwan.
Colloids Surf B Biointerfaces. 2017 Mar 1;151:372-383. doi: 10.1016/j.colsurfb.2016.12.040. Epub 2016 Dec 29.
This work discusses the impact of the charge bias and the hydrophilicity on the human blood compatibility of pseudozwitterionic biomaterial gels. Four series of hydrogels were prepared, all containing negatively-charged 3-sulfopropyl methacrylate (SA), and either acrylamide, N-isopropylacrylamide, 2-dimethylaminoethyl methacrylate (DMAEMA) or [2-(methacryloyloxy)ethyl]trimethylammonium (TMA), to form SA, SN, SD or ST hydrogels, respectively. An XPS analysis proved that the polymerization was well controlled from the initial monomer ratios. All gels present high surface hydrophilicity, but varying bulk hydration, depending on the nature/content of the comonomer, and on the immersion medium. The most negative interfaces (pure SA, S7A3, S5A5) showed significant fibrinogen adsorption, ascribed to the interactions of the αC domains of the protein with the gels, then correlated to considerable platelet adhesion; but low leukocyte/erythrocyte attachments were measured. Positive gels (excess of DMAEMA or TMA) are not hemocompatible. They mediate protein adsorption and the adhesion of human blood cells, through electrostatic attractive interactions. The neutral interfaces (zeta potential between -10mV and +10mV) are blood-inert only if they present a high surface and bulk hydrophilicity. Overall, this study presents a map of the hemocompatible behavior of hydrogels as a function of their surface charge-bias, essential to the design of blood-contacting devices.
这项工作讨论了电荷偏置和亲水性对假两性离子生物材料凝胶的人体血液相容性的影响。制备了四个系列的水凝胶，所有水凝胶都含有带负电荷的甲基丙烯酸3-磺丙酯（SA），以及丙烯酰胺、N-异丙基丙烯酰胺、甲基丙烯酸2-二甲氨基乙酯（DMAEMA）或[2-(甲基丙烯酰氧基)乙基]三甲基铵（TMA），分别形成SA、SN、SD或ST水凝胶。X射线光电子能谱分析证明，聚合反应从初始单体比例开始就得到了很好的控制。所有凝胶都具有高表面亲水性，但根据共聚单体的性质/含量以及浸泡介质的不同，其本体水合程度有所变化。最负的界面（纯SA、S7A3、S5A5）显示出显著的纤维蛋白原吸附，这归因于蛋白质的αC结构域与凝胶的相互作用，进而与大量血小板粘附相关；但白细胞/红细胞附着量较低。带正电的凝胶（DMAEMA或TMA过量）不具有血液相容性。它们通过静电吸引相互作用介导蛋白质吸附和人体血细胞的粘附。中性界面（zeta电位在-10mV至+10mV之间）只有在具有高表面和本体亲水性时才对血液无活性。总体而言，本研究给出了水凝胶血液相容性行为随其表面电荷偏置变化的图谱，这对血液接触装置的设计至关重要。
