Venault Antoine, Zheng Yong-Sheng, Chinnathambi Arunachalam, Alharbi Sulaiman Ali, Ho Hsin-Tsung, Chang Yu, Chang Yung
R&D Center for Membrane Technology and Department of Chemical Engineering, Chung Yuan Christian University , Jhong-Li, Taoyuan 320, Taiwan.
Langmuir. 2015 Mar 10;31(9):2861-9. doi: 10.1021/la505000m. Epub 2015 Feb 24.
We report a novel biomacromolecular formula for the design of hemocompatible gel interfaces of N-isopropylacrylamide (NIPAAm) and mixed-charge pairs of [2-(methacryloyloxy)ethyl]trimethylammonium (TMA) and 3-sulfopropyl methacrylate (SA) with overall electrical neutrality. The study stresses on how well-defined compositions of nonionic NIPAAm and pseudozwitterionic TMA/SA in the poly(NIPAAm-co-TMA/SA) hydrogels along with environmental conditions (temperature, ionic strength, and solution pH) affect swelling and adhesion of biofoulants on their surfaces. When challenged with plasma proteins, bacteria, recalcified platelets, or whole blood, stimuli-responsive hydrogels better resisted their adhesion as the content of mixed charges in the copolymer increased, to reach nonbiofouling for the gels made of 100% TMA/SA. The low hemolytic activity (0.5%) associated with a long plasma clotting time (10 min) suggests excellent hemocompatibility excellent hemocompatibility. Finally, hydrogels containing both NIPAAm and TMA/SA tend to exhibit preferential adhesion of leukocytes.
我们报道了一种新型生物大分子配方，用于设计具有血液相容性的凝胶界面，该凝胶由N-异丙基丙烯酰胺（NIPAAm）以及[2-(甲基丙烯酰氧基)乙基]三甲基铵（TMA）和甲基丙烯酸3-磺丙酯（SA）的混合电荷对组成，整体呈电中性。该研究着重探讨了聚（NIPAAm-co-TMA/SA）水凝胶中明确的非离子型NIPAAm和假两性离子型TMA/SA的组成，以及环境条件（温度、离子强度和溶液pH值）如何影响生物污垢在其表面的膨胀和附着。当受到血浆蛋白、细菌、复钙血小板或全血挑战时，随着共聚物中混合电荷含量的增加，刺激响应性水凝胶对它们的附着具有更好的抵抗力，对于由100% TMA/SA制成的凝胶可实现无生物污垢。低溶血活性（0.5%）与较长的血浆凝固时间（10分钟）表明具有优异的血液相容性。最后，含有NIPAAm和TMA/SA的水凝胶往往表现出对白细胞的优先附着。
