Jhong Jheng-Fong, Sin Mei-Chan, Kung Hsiao-Han, Chinnathambi Arunachalam, Alharbi Sulaiman Ali, Chang Yung
a Department of Chemical Engineering and R&D Center for Membrane Technology , Chung Yuan Christian University , Jhong-Li , Taoyuan 320 , Taiwan.
J Biomater Sci Polym Ed. 2014;25(14-15):1558-72. doi: 10.1080/09205063.2014.921754. Epub 2014 Jun 4.
In this study, a pseudozwitterionic surface bearing positively and negatively mixed charged moieties was developed as a potential hemocompatible material for biomedical applications. In this work, hemocompatility of pseudozwitterionic surface prepared from copolymerization of negatively charged 3-sulfopropyl methacrylate (SA) and positively charged [2-(methacryloyloxy)ethyl] trimethylammonium (TMA) was delineated. Mixed charge distribution in the prepared poly(TMA-co-SA)-grafted surface can be controlled by regulating TMA and SA monomer ratios via surface-initiated atom transfer radical polymerization. The effects of grafting composition and charge bias variations on blood compatibility of poly(TMA-co-SA)-grafted surface were reported. The protein adsorption on different poly(TMA-co-SA)-grafted surfaces from human plasma protein (fibrinogen, HSA, and γ-globulin) solutions was evaluated using an enzyme-linked immunosorbent assay. Blood platelet adhesion and time measurements on plasma clotting were conducted to determine the platelet activation on the grafted surface. It was found that the protein resistance and anti-blood cell adhesion of prepared surface can be precisely controlled by controlling the charge balance of TMA/SA compositions. In addition, different charge bias variations on the poly(TMA-co-SA)-grafted surface would induce electrostatic interactions between plasma proteins and prepared surfaces which lead to adsorptions of interfacial protein and blood cells, plasma clotting, and blood cell hemolysis. Results from this study suggest that the hemocompatility of mixed charged poly(TMA-co-SA)-grafted surface depends on the charge bias level. This provides a great potential for designing biomaterial with unique surface chemical structure which could be used in contact with human blood.
在本研究中，开发了一种带有正负混合电荷部分的假两性离子表面，作为一种潜在的用于生物医学应用的血液相容性材料。在这项工作中，描述了由带负电荷的甲基丙烯酸3 - 磺丙酯（SA）和带正电荷的[2 - （甲基丙烯酰氧基）乙基]三甲基氯化铵（TMA）共聚制备的假两性离子表面的血液相容性。通过表面引发的原子转移自由基聚合调节TMA和SA单体比例，可以控制制备的聚（TMA - co - SA）接枝表面的混合电荷分布。报道了接枝组成和电荷偏置变化对聚（TMA - co - SA）接枝表面血液相容性的影响。使用酶联免疫吸附测定法评估了不同聚（TMA - co - SA）接枝表面对人血浆蛋白（纤维蛋白原、HSA和γ - 球蛋白）溶液的蛋白质吸附。进行了血小板粘附和血浆凝血时间测量，以确定接枝表面上的血小板活化情况。发现通过控制TMA/SA组合物的电荷平衡，可以精确控制制备表面的蛋白质抗性和抗血细胞粘附性。此外，聚（TMA - co - SA）接枝表面上不同的电荷偏置变化会诱导血浆蛋白与制备表面之间的静电相互作用，从而导致界面蛋白和血细胞的吸附、血浆凝血和血细胞溶血。本研究结果表明，混合电荷的聚（TMA - co - SA）接枝表面的血液相容性取决于电荷偏置水平。这为设计具有独特表面化学结构的生物材料提供了巨大潜力，该生物材料可用于与人体血液接触。
