Lauritsen K Tølbøll, Hagedorn-Olsen T, Jungersen G, Riber U, Stryhn H, Friis N F, Lind P, Kristensen B
National Veterinary Institute, Technical University of Denmark, Bülowsvej 27, 1870 Frederiksberg C, Denmark; Department of Disease Biology, Faculty of Health and Medical Sciences, University of Copenhagen, Stigbøjlen 4, 1870 Frederiksberg C, Denmark.
National Veterinary Institute, Technical University of Denmark, Bülowsvej 27, 1870 Frederiksberg C, Denmark.
Vet Immunol Immunopathol. 2017 Jan;183:22-30. doi: 10.1016/j.vetimm.2016.12.002. Epub 2016 Dec 5.
Mycoplasma hyosynoviae causes arthritis in pigs older than 12 weeks. The role of colostrum in protection of piglets against M. hyosynoviae infection is not clear. Our objective was therefore to investigate whether transfer of maternal immunity to piglets was involved in early protection against the infection. Experimental infections were carried out in three groups of weaners receiving different levels of M. hyosynoviae-specific colostrum components; Group NC derived from Mycoplasma free sows and possessed no specific immunity to M. hyosynoviae. Group CAb pigs, siblings of the NC group, received colostrum with M. hyosynoviae-specific antibodies immediately after birth. Group CCE pigs were born and raised by infected sows and presumably had the full set of colostrally transferred factors, including specific antibodies. When 4½ weeks old, all pigs were inoculated intranasally with M. hyosynoviae. The course of infection was measured through clinical observations of lameness, cultivation of M. hyosynoviae from tonsils, blood and synovial fluid and observation for gross pathological lesions in selected joints. Specific immune status in the pigs was evaluated through detection of antibodies by immunoblotting and measurement of M. hyosynoviae-specific T-cell proliferation. The latter analysis may possibly indicate that M. hyosynoviae infection induces a T-cell response. The CCE piglets were significantly protected against development of lameness and pathology, as well as infection with M. hyosynoviae in tonsils, blood and joints, when compared to the two other groups. Raising the CCE pigs in an infected environment until weaning, with carrier sows as mothers, apparently made them resistant to M. hyosynoviae-arthritis when challenge-infected at 4½ weeks of age. More pigs in group NC had M. hyosynoviae related pathological lesions than in group CAb, a difference that was significant for cubital joints when analysed on joint type level. This finding indicates a partially protective effect of passively transferred M. hyosynoviae-specific colostral antibodies upon development of M. hyosynoviae related pathology. Thus, the level of passive immunity transferred from sow to piglet seems to provide, at least partial, protection against development of arthritis. It cannot be ruled out that the CCE pigs, by growing up in an infected environment, have had the chance to establish an active anti-M. hyosynoviae immune response that complements the maternally transferred immune factors. Evident from this study is that the general absence of M. hyosynoviae arthritis in piglets can be ascribed mainly to their immunological status.
猪滑膜支原体可导致12周龄以上的猪发生关节炎。初乳在保护仔猪抵抗猪滑膜支原体感染方面的作用尚不清楚。因此，我们的目的是研究母体免疫向仔猪的传递是否参与了对该感染的早期保护。对三组断奶仔猪进行了实验性感染，它们接受了不同水平的猪滑膜支原体特异性初乳成分；NC组仔猪来自无支原体的母猪，对猪滑膜支原体没有特异性免疫力。CAb组仔猪是NC组的同胞，出生后立即接受了含有猪滑膜支原体特异性抗体的初乳。CCE组仔猪由感染母猪所生并饲养，可能拥有全套通过初乳传递的因子，包括特异性抗体。当仔猪4.5周龄时，所有仔猪均经鼻接种猪滑膜支原体。通过对跛行的临床观察、从扁桃体、血液和滑液中培养猪滑膜支原体以及观察选定关节的大体病理损伤来测定感染过程。通过免疫印迹法检测抗体以及测量猪滑膜支原体特异性T细胞增殖来评估仔猪的特异性免疫状态。后一项分析可能表明猪滑膜支原体感染会诱导T细胞反应。与其他两组相比，CCE组仔猪在跛行、病理变化以及扁桃体、血液和关节感染猪滑膜支原体方面受到了显著保护。将CCE组仔猪在感染环境中饲养至断奶，以携带病原体的母猪为母体，显然使它们在4.5周龄时受到攻击感染时对猪滑膜支原体性关节炎具有抵抗力。NC组中出现猪滑膜支原体相关病理损伤的仔猪比CAb组更多，在按关节类型水平分析时，这一差异在肘关节方面具有显著性。这一发现表明，被动传递的猪滑膜支原体特异性初乳抗体对猪滑膜支原体相关病理变化的发展具有部分保护作用。因此，从母猪传递给仔猪的被动免疫水平似乎至少能部分地保护仔猪不发生关节炎。不能排除CCE组仔猪由于在感染环境中成长，有机会建立起一种积极的抗猪滑膜支原体免疫反应，从而补充了母体传递的免疫因子。从这项研究中可以明显看出，仔猪普遍不存在猪滑膜支原体关节炎主要可归因于它们的免疫状态。
