Leódido Ana Carolina M, Costa Luis E C, Araújo Thiago S L, Costa Douglas S, Sousa Nayara A, Souza Luan K M, Sousa Francisca B M, Filho Manoel D S, Vasconcelos Daniel F P, Silva Felipe R P, Nogueira Kerolayne M, Araújo Alyne R, Barros Francisco Clark N, Freitas Ana Lúcia P, Medeiros Jand Venes R
The Northeast Biotechnology Network (RENORBIO), Federal University of Piauí, Teresina, PI 64049-550, Brazil.
Laboratory of Proteins and Carbohydrates of Marine Algae, Department of Biochemistry and Molecular Biology, Federal University of Ceará, Fortaleza, CE 60455-760, Brazil.
Int J Biol Macromol. 2017 Apr;97:34-45. doi: 10.1016/j.ijbiomac.2017.01.006. Epub 2017 Jan 5.
Sulphated polysaccharides extracted from algae have been extensively studied for their diverse biological activities. Thus, the purpose of this study was to evaluate the chemical composition, the anti-diarrhoeal effect and acute toxicity of a sulphated polysaccharide fraction obtained from Gracilaria intermedia (SP-Gi). Initially, the FT-IR of SP-Gi revealed to be an agaran with sulphation at C-6 of the l-galactosyl residues. The anti-diarrhoeal activity of SP-Gi was evaluated in a castor oil-induced diarrhoea model. The effects of SP-Gi on enteropooling, Na -K -ATPase activity, gastrointestinal transit, and gastric emptying were then examined. Subsequently, the effect of SP-Gi on diarrhoea induced by cholera toxin (CT) and Escherichia coli was examined. In addition, an acute toxicity test was conducted in accordance with OECD guideline 423. Pre-treatment with SP-Gi reduces the total faeces, total diarrhoeal faeces, and enteropooling. SP-Gi (30mg/kg p.o.) increased Na/K-ATPase activity and reduced gastrointestinal transit through anticholinergic mechanisms. ELISA demonstrated that SP-Gi can interact with GM1 receptors and CT. SP-Gi reduced diarrhoea induced by E. coli and prevented weight loss in the animals. Moreover, SP-Gi did not induce any toxicity signs. These results suggest that SP-Gi is a possible candidate for the treatment of diarrhoeal illnesses.
从藻类中提取的硫酸化多糖因其多样的生物活性而受到广泛研究。因此，本研究的目的是评估从江蓠（SP-Gi）中获得的硫酸化多糖组分的化学成分、抗腹泻作用和急性毒性。最初，SP-Gi的傅里叶变换红外光谱显示其为一种在l-半乳糖基残基的C-6位硫酸化的琼脂糖。在蓖麻油诱导的腹泻模型中评估了SP-Gi的抗腹泻活性。然后研究了SP-Gi对肠积液、钠钾ATP酶活性、胃肠转运和胃排空的影响。随后，研究了SP-Gi对霍乱毒素（CT）和大肠杆菌诱导的腹泻的影响。此外，按照经合组织准则423进行了急性毒性试验。用SP-Gi预处理可减少粪便总量、腹泻粪便总量和肠积液。SP-Gi（30mg/kg口服）通过抗胆碱能机制增加钠/钾ATP酶活性并减少胃肠转运。酶联免疫吸附测定表明SP-Gi可与GM1受体和CT相互作用。SP-Gi减轻了大肠杆菌诱导的腹泻并防止动物体重减轻。此外，SP-Gi未引起任何毒性迹象。这些结果表明SP-Gi可能是治疗腹泻疾病的候选药物。
