Mahdavinia Gholam Reza, Mosallanezhad Amirabbas, Soleymani Moslem, Sabzi Mohammad
Polymer Research Laboratory, Department of Chemistry, Faculty of Science, University of Maragheh, P.O. Box 55181-83111, Maragheh, Iran.
Department of Materials Engineering, Faculty of Engineering, University of Maragheh, P.O. Box 55181-83111, Maragheh, Iran.
Int J Biol Macromol. 2017 Apr;97:209-217. doi: 10.1016/j.ijbiomac.2017.01.012. Epub 2017 Jan 4.
The aim of the present work was to develop green carriers for methotrexate using κ-carrageenan/chitosan complexes. Magnetic FeO nanoparticles were first synthesized in the presence of κ-carrageenan through in situ method. Then, the obtained magnetic κ-carrageenan was crosslinked using the polycation chitosan biopolymer. The physical and structural properties of hydrogels were investigated by FTIR, XRD, SEM, TEM, TGA, and VSM techniques. The pH-dependent swelling behavior of hydrogels was examined in various buffer solutions. All of the prepared hydrogels showed a high swelling capacity in basic solutions. The introduction of magnetite nanoparticles into κ-carrageenan/chitosan complexes had a significant effect on the swelling capacity of magnetic hydrogels, as the water absorbency of hydrogels decreased with increasing magnetite content. Methotrexate as an anticancer and model drug was loaded on hydrogels and the release profiles were investigated at pH=7.4 and 5.3. The methotrexate encapsulation efficiency was increased by increasing magnetite and chitosan contents. The results demonstrated that the release of methotrexate from magnetic hydrogels is pH-dependent with a high release content at pH=7.4. The release profiles were analyzed by Peppas's empirical model and the release of drug from hydrogels followed Fickian type of diffusion mechanism at both pHs.
本研究的目的是利用κ-卡拉胶/壳聚糖复合物开发甲氨蝶呤的绿色载体。首先通过原位法在κ-卡拉胶存在下合成磁性FeO纳米颗粒。然后，使用聚阳离子壳聚糖生物聚合物对获得的磁性κ-卡拉胶进行交联。通过傅里叶变换红外光谱（FTIR）、X射线衍射（XRD）、扫描电子显微镜（SEM）、透射电子显微镜（TEM）、热重分析（TGA）和振动样品磁强计（VSM）技术研究水凝胶的物理和结构性质。在各种缓冲溶液中考察水凝胶的pH依赖性溶胀行为。所有制备的水凝胶在碱性溶液中均表现出高溶胀能力。将磁铁矿纳米颗粒引入κ-卡拉胶/壳聚糖复合物对磁性水凝胶的溶胀能力有显著影响，随着磁铁矿含量的增加，水凝胶的吸水率降低。将甲氨蝶呤作为抗癌模型药物负载在水凝胶上，并在pH = 7.4和5.3条件下研究其释放曲线。通过增加磁铁矿和壳聚糖的含量提高了甲氨蝶呤的包封效率。结果表明，磁性水凝胶中甲氨蝶呤的释放具有pH依赖性，在pH = 7.4时释放量较高。通过Peppas经验模型分析释放曲线，在两个pH值下，药物从水凝胶中的释放均遵循菲克型扩散机制。
