School of Chemical Engineering, Shandong University of Technology, Zibo 255049, PR China.
School of Chemical Engineering, Shandong University of Technology, Zibo 255049, PR China.
Biosens Bioelectron. 2017 May 15;91:441-448. doi: 10.1016/j.bios.2016.12.070. Epub 2016 Dec 31.
In this work, a sensitive sandwich-type electrochemical immunosensor was designed for the quantitative detection of prostate-specific antigen (PSA) by amperometric i-t. The Au loaded on thionine functionalized graphene oxide (Au@Th/GO) was used as a platform to immobilize primary antibodies (Ab) and accelerate the electron transfer on the electrode interface. PtCu bimetallic hybrid were loaded on 2D/2D reduced graphene oxide/graphitic carbon nitride (PtCu@rGO/g-CN) with large surface area and biocompatibility, which were employed as labels for combining secondary antibodies (Ab) and amplifying signals to improve the sensitivity of the designed immunosensor which attributes to its good activity for the reduction of hydrogen peroxide (HO). Under optimal conditions, the designed immunosensor exhibited a linear concentration range from 50fg/mL to 40ng/mL, with a low detection limit of 16.6fg/mL (S/N=3) for PSA. Additionally, the designed immunosensor showed acceptable selectivity, reproducibility and stability. The satisfactory results in analyze human serum samples indicated potential application promising in clinical monitoring of tumor markers.
在这项工作中，通过安培 i-t 设计了一种灵敏的三明治型电化学免疫传感器，用于前列腺特异性抗原 (PSA) 的定量检测。负载在噻吩功能化氧化石墨烯 (Au@Th/GO) 上的 Au 被用作固定化一级抗体 (Ab) 的平台，并加速电极界面上的电子转移。具有大表面积和生物相容性的二维/二维还原氧化石墨烯/石墨相氮化碳 (PtCu@rGO/g-CN) 上负载了 PtCu 双金属杂化物，其被用作结合二级抗体 (Ab) 和放大信号的标记物，以提高设计的免疫传感器的灵敏度，这归因于其对过氧化氢 (HO) 的还原具有良好的活性。在最佳条件下，设计的免疫传感器表现出从 50fg/mL 到 40ng/mL 的线性浓度范围，对 PSA 的检测限低至 16.6fg/mL（S/N=3）。此外，设计的免疫传感器表现出可接受的选择性、重现性和稳定性。在分析人血清样本中的令人满意的结果表明，它在肿瘤标志物的临床监测中有潜在的应用前景。
