Søe Niels H, Jensen Nina Vendel, Jensen Asger Lundorff, Koch Janne, Poulsen Steen Seier, Pier Gerald B, Johansen Helle Krogh
Hand Section, Department of Orthopaedics, Herlev and Gentofte University Hospital, Hellerup, Denmark
Department of Anaesthesiology, Intensive Care and Operations, Herlev and Gentofte University Hospital, Hellerup, Denmark.
In Vivo. 2017 Jan 2;31(1):45-50. doi: 10.21873/invivo.11023.
BACKGROUND/AIM: Staphylococcus aureus infection associated with orthopedic implants cannot always be controlled. We used a knee prosthesis model with implant-related osteomyelitis in rats to explore induction of an effective immune response with active and passive immunization.
Fifty-two Sprague-Dawley rats were divided into active (N=28) and passive immunization groups (N=24). A bacterial inoculum of 10 S. aureus MN8 was injected into the tibia and the femur marrow before insertion of a non-constrained knee prosthesis in each rat. The active-immunization group received a synthetic oligosaccharide of polysaccharide poly-N-acetylglucosamine (PNAG), 9G1cNH and the passive-immunization group received immunization with immunoglobulin from rabbits infected with S. aureus.
RESULTS/CONCLUSION: Active immunization against PNAG significantly reduced the consequences of osteomyelitis infection from PNAG-producing intercellular adhesion (ica) but not ica S. aureus. Passive immunization resulted in better clinical assessments in animals challenged with either ica or ica S. aureus, suggesting a lack of specificity in this antiserum.
背景/目的：与骨科植入物相关的金黄色葡萄球菌感染并非总能得到控制。我们使用大鼠植入物相关骨髓炎的膝关节假体模型，通过主动免疫和被动免疫来探索诱导有效的免疫反应。
将52只斯普拉格-道利大鼠分为主动免疫组（n = 28）和被动免疫组（n = 24）。在每只大鼠植入非限制性膝关节假体之前，将10个金黄色葡萄球菌MN8的菌悬液注入胫骨和股骨骨髓。主动免疫组接受多糖聚-N-乙酰葡糖胺（PNAG）的合成寡糖9G1cNH免疫，被动免疫组接受来自感染金黄色葡萄球菌的兔子的免疫球蛋白免疫。
结果/结论：针对PNAG的主动免疫显著减轻了由产生PNAG的细胞间黏附（ica）而非ica金黄色葡萄球菌引起的骨髓炎感染后果。被动免疫在接受ica或ica金黄色葡萄球菌攻击的动物中产生了更好的临床评估结果，这表明该抗血清缺乏特异性。
