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利用Affymetrix基因芯片阵列分析钕钇铝石榴石激光照射后培养的大鼠内皮细胞的基因表达

Gene Expression Analysis of Cultured Rat-Endothelial Cells after Nd:YAG Laser Irradiation by Affymetrix GeneChip Array.

作者信息

Masuda Yoshiko, Yokose Satoshi, Sakagami Hiroshi

机构信息

Meikai Pharmaco-Medical Laboratory (MPL), Meikai University School of Dentistry, Saitama, Japan

Division of Endodontics and Operative, Meikai University School of Dentistry, Saitama, Japan.

出版信息

In Vivo. 2017 Jan 2;31(1):51-54. doi: 10.21873/invivo.11024.

DOI:10.21873/invivo.11024
PMID:28064220
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5354147/
Abstract

Endothelial cells and dental pulp cells enhance osteo-/odontogenic and angiogenic differentiation. In our previous study, rat pulp cells migrated to Nd:YAG laser-irradiated endothelial cells in an insert cell culture system. The purpose of this study was to examine the possible changes in the gene expression of cultured rat aortic endothelial cells after Nd:YAG laser irradiation using affymetrix GeneChip Array. Total RNA was extracted from the cells at 5 h after laser irradiation. Gene expressions were evaluated by DNA array chip. Up-regulated genes were related to cell migration and cell structure (membrane stretch, actin regulation and junctional complexes), neurotransmission and inflammation. Heat-shock 70 kDa protein (Hsp70) was related to the development of tooth germ. This study offers candidate genes for understanding the relationship between the laser-stimulated endothelial cells and dental pulp cells.

摘要

内皮细胞和牙髓细胞可增强骨/牙源性及血管生成分化。在我们之前的研究中，在插入式细胞培养系统中，大鼠牙髓细胞迁移至钕:钇铝石榴石（Nd:YAG）激光照射的内皮细胞。本研究的目的是使用Affymetrix基因芯片阵列检测Nd:YAG激光照射后培养的大鼠主动脉内皮细胞基因表达的可能变化。激光照射后5小时从细胞中提取总RNA。通过DNA阵列芯片评估基因表达。上调的基因与细胞迁移和细胞结构（膜伸展、肌动蛋白调节和连接复合体）、神经传递及炎症相关。热休克70 kDa蛋白（Hsp70）与牙胚发育有关。本研究为理解激光刺激的内皮细胞与牙髓细胞之间的关系提供了候选基因。

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