白细胞介素13对骨关节炎中滑膜细胞骨膜蛋白分泌的影响
Influence of IL13 on Periostin Secretion by Synoviocytes in Osteoarthritis.
作者信息
Moue Tatsuya, Tajika Yutaro, Ishikawa Shintaro, Kanada Yasuaki, Okumo Takayuki, Asano Kazuhito, Hisamitsu Tadashi
机构信息
Department of Physiology, School of Medicine, Showa University, Tokyo, Japan.
Department of Physiology, School of Medicine, Showa University, Tokyo, Japan
出版信息
In Vivo. 2017 Jan 2;31(1):79-85. doi: 10.21873/invivo.11028.
BACKGROUND
Our previous research provided evidence of periostin increase in parallel with interleukin-13 (IL13) increase in the synovial fluid of patients with osteoarthritis (OA). The reaction cascade from IL13 to periostin, however, remains unidentified. We, therefore, tested the hypothesis that periostin secretion is affected downstream of IL13.
MATERIALS AND METHODS
OA synoviocytes were cultured under different concentrations of IL13. Periostin content in culture supernatants and the level of signal transducer and activator of transcription 6 (STAT6) in the cultured cells were measured using enzyme-linked immunosorbent assay (ELISA). Moreover, the influence of dexamethasone and leflunomide on periostin production in relation to the effect of IL13 on the cells was also examined.
RESULTS
Periostin content in culture supernatants and the level of STAT6 in cultured cells were significantly increased by IL13. The increase of periostin was significantly inhibited by dexamethasone and leflunomide.
CONCLUSION
Periostin may be up-regulated in OA synoviocytes via STAT6 downstream of IL13.
背景
我们之前的研究表明，骨膜蛋白的增加与骨关节炎（OA）患者滑液中白细胞介素-13（IL13）的增加平行。然而，从IL13到骨膜蛋白的反应级联仍未明确。因此，我们检验了骨膜蛋白分泌受IL13下游影响的假设。
材料与方法
在不同浓度的IL13下培养OA滑膜细胞。使用酶联免疫吸附测定（ELISA）测量培养上清液中的骨膜蛋白含量以及培养细胞中信号转导和转录激活因子6（STAT6）的水平。此外，还研究了地塞米松和来氟米特对骨膜蛋白产生的影响以及它们与IL13对细胞作用的关系。
结果
IL13显著增加了培养上清液中的骨膜蛋白含量以及培养细胞中STAT6的水平。地塞米松和来氟米特显著抑制了骨膜蛋白的增加。
结论
骨膜蛋白可能通过IL13下游的STAT6在OA滑膜细胞中上调。
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