Kobayashi S, Kasuya M, Itoi M
Kyoritsu College of Pharmacy, Tokyo, Japan.
Exp Eye Res. 1989 Oct;49(4):553-9. doi: 10.1016/s0014-4835(89)80054-5.
Changes in lens proteins induced at the early stages of cataractogenesis in cac (Nakano) mice were investigated by two-dimensional gel electrophoresis (2D-PAGE). The 2D-PAGE profile of lens proteins in 21-day-old cac mice differed from that in 27-day-old normal mice, even though the appearance of 'pin-head' nuclear opacity (26-day-old) had not yet been observed in the lenses. Especially noticeable were great differences in the polypeptides associated with the alpha- and beta- crystallin subfractions, the appearances of which corresponded to an increase in a ratio of the amounts of Na+ to that of K+ in the lenses of defective mice. No dramatic decrease in the gamma-crystallin fraction was observed until the mature cataract stage.
通过二维凝胶电泳（2D-PAGE）研究了cac（中野）小鼠白内障发生早期诱导的晶状体蛋白变化。21日龄cac小鼠晶状体蛋白的二维电泳图谱与27日龄正常小鼠不同，尽管在晶状体中尚未观察到“针头状”核混浊（26日龄）的出现。特别值得注意的是，与α-和β-晶状体蛋白亚组分相关的多肽存在很大差异，其出现与缺陷小鼠晶状体中Na+与K+含量之比的增加相对应。直到成熟白内障阶段才观察到γ-晶状体蛋白组分的显著减少。
