Shinohara T, Piatigorsky J
Science. 1980 Nov 21;210(4472):914-6. doi: 10.1126/science.7434006.
In vitro translation experiments showed that the lens fiber cells of two hereditary cataracts in mice (Nakano and Philly) possessed a full complement of crystallin messenger RNA's, despite severely reduced synthesis of crystallin in these cells. The reduction in synthesis in the lens fiber cells correlated with the increase in Na+ and the decrease in K+, which occurs during cataractogenesis. In contrast to the fiber cells, the epithelial cells continued to synthesize crystallins in the cataractous lenses. Crystallin synthesis was stimulated in the fiber cells by raising the K+ concentration and lowering the Na+ concentration in the cultured lenses. The reduction in crystallin synthesis in the initial stages of cataractogenesis in the Nakano and Philly lenses thus appears to be due to poor utilization of crystallin messenger RNA's in the fiber cells.
体外翻译实验表明，小鼠中两种遗传性白内障（中野和费城型）的晶状体纤维细胞拥有完整的晶状体蛋白信使核糖核酸，但这些细胞中晶状体蛋白的合成却严重减少。晶状体纤维细胞中合成的减少与白内障形成过程中发生的钠离子增加和钾离子减少相关。与纤维细胞不同，上皮细胞在白内障晶状体中继续合成晶状体蛋白。通过提高培养晶状体中的钾离子浓度并降低钠离子浓度，可刺激纤维细胞中的晶状体蛋白合成。因此，中野和费城型晶状体在白内障形成初始阶段晶状体蛋白合成的减少似乎是由于纤维细胞中晶状体蛋白信使核糖核酸的利用不佳所致。
