人诱导磷灰石形成的主动脉蛋白脂质的分离与特性分析

Isolation and characterization of human apatite-inducing aortic proteolipid.

作者信息

Romeo R, Augustyn J M, Mandel G

机构信息

Department of Pathology, Albany Medical College, New York 12208.

出版信息

Exp Mol Pathol. 1989 Oct;51(2):149-58. doi: 10.1016/0014-4800(89)90015-4.

DOI:10.1016/0014-4800(89)90015-4

PMID:2806469

Abstract

A proteolipid, capable of inducing hydroxyapatite in vitro, can be isolated from human aortic calcified lesions, noncalcified lesions, and nonlesion tissue obtained at autopsy. Analysis of the amino acid composition after acid hydrolysis showed all nucleators to be high in the hydrophobic amino acids glycine and alanine. Estimation of the molecular weight of the nucleator, extracted from calcified lesions, by gel filtration and lipid content showed a minimum molecular weight of 469,000 Da. All nucleators were characterized by the presence of acidic phospholipids which may have a role in the mechanism of calcification.

摘要

一种能够在体外诱导羟基磷灰石形成的蛋白脂质，可以从人体主动脉钙化病变组织、非钙化病变组织以及尸检获得的非病变组织中分离出来。酸水解后对氨基酸组成的分析表明，所有成核剂中疏水性氨基酸甘氨酸和丙氨酸的含量都很高。通过凝胶过滤和脂质含量对从钙化病变中提取的成核剂分子量进行估计，结果显示其最小分子量为469,000道尔顿。所有成核剂的特征均为含有酸性磷脂，这可能在钙化机制中发挥作用。