Ennever J, Vogel J J, Riggan L J
Atherosclerosis. 1980 Feb;35(2):209-13. doi: 10.1016/0021-9150(80)90085-4.
Calcified atherosclerotic aorta was examined for proteolipid capable of nucleating apatite, the crystal species of aortic calcification. Appropriate tissue pieces were decalcified with dilute formic acid and extracted with chloroform-methanol. Lipid fractionation yielded proteolipid which, upon incubation in metastable calcium phosphate solution, induced apatite crystallization. The proteolipid was partially characterized as a hydrophobic protein, acidic phospholipid complex. It resembles the nucleator previously demonstrated for bone matrix calcification.
对钙化的动脉粥样硬化主动脉进行检查，寻找能够使磷灰石成核的蛋白脂质，磷灰石是主动脉钙化的晶体种类。将合适的组织块用稀甲酸脱钙，并用氯仿 - 甲醇提取。脂质分级分离得到蛋白脂质，该蛋白脂质在亚稳态磷酸钙溶液中孵育时会诱导磷灰石结晶。该蛋白脂质部分被表征为一种疏水蛋白与酸性磷脂的复合物。它类似于先前在骨基质钙化中所证实的成核剂。
