动脉粥样硬化主动脉中蛋白脂质导致的钙化。

Calcification by proteolipid from atherosclerotic aorta.

作者信息

Ennever J, Vogel J J, Riggan L J

出版信息

Atherosclerosis. 1980 Feb;35(2):209-13. doi: 10.1016/0021-9150(80)90085-4.

Abstract

Calcified atherosclerotic aorta was examined for proteolipid capable of nucleating apatite, the crystal species of aortic calcification. Appropriate tissue pieces were decalcified with dilute formic acid and extracted with chloroform-methanol. Lipid fractionation yielded proteolipid which, upon incubation in metastable calcium phosphate solution, induced apatite crystallization. The proteolipid was partially characterized as a hydrophobic protein, acidic phospholipid complex. It resembles the nucleator previously demonstrated for bone matrix calcification.

摘要

对钙化的动脉粥样硬化主动脉进行检查，寻找能够使磷灰石成核的蛋白脂质，磷灰石是主动脉钙化的晶体种类。将合适的组织块用稀甲酸脱钙，并用氯仿 - 甲醇提取。脂质分级分离得到蛋白脂质，该蛋白脂质在亚稳态磷酸钙溶液中孵育时会诱导磷灰石结晶。该蛋白脂质部分被表征为一种疏水蛋白与酸性磷脂的复合物。它类似于先前在骨基质钙化中所证实的成核剂。