动态活体成像观察淋巴结中的细胞-细胞相互作用。

Dynamic intravital imaging of cell-cell interactions in the lymph node.

机构信息

Theodor Kocher Institute, University of Bern, Bern, Switzerland.

Institute for Research in Biomedicine, Università della Svizzera Italiana, Bellinzona, Switzerland.

出版信息

J Allergy Clin Immunol. 2017 Jan;139(1):12-20. doi: 10.1016/j.jaci.2016.11.008.

DOI:10.1016/j.jaci.2016.11.008

PMID:28065277

Abstract

In the last decade, the application of 2-photon intravital microscopy as a tool to study cell interactions in different areas of the immune system has offered an unprecedented opportunity to understand the complexity of cell behavior in relation to immune functions. In this review we describe the latest advances in the field of live imaging in the lymph nodes, grouping the different cell populations in 2 compartments according to their motility: the sessile compartment, which is formed by resident cells of stromal origin, macrophages, and resident dendritic cells, and the motile compartment, which is mainly formed by T and B lymphocytes. Here we review how the use of in vivo imaging has contributed to our understanding of the role of these cells in the initiation of the immune response in the draining lymph nodes.

摘要

在过去的十年中，双光子活体显微镜作为一种研究免疫系统不同区域细胞相互作用的工具，为理解细胞行为与免疫功能之间的复杂性提供了前所未有的机会。在这篇综述中，我们根据其运动性将淋巴结内活体成像领域的最新进展分组描述不同的细胞群体，分为两个隔室：固定细胞隔室，由基质来源的常驻细胞、巨噬细胞和常驻树突状细胞组成，以及运动细胞隔室，主要由 T 和 B 淋巴细胞组成。在这里，我们回顾了体内成像的使用如何有助于我们理解这些细胞在引流淋巴结中免疫反应启动中的作用。