Maruthiah Thirumalai, Palavesam Arunachalam
Centre for Marine Science and Technology, Manonmaniam Sundaranar University, Rajakkamangalam-629 502, Kanyakumari District, Tamilnadu, India.
Department of Animal Science, Manonmaniam Sundaranar University, Tirunelveli 627 012, Tamilnadu, India.
Int J Biol Macromol. 2017 Apr;97:552-560. doi: 10.1016/j.ijbiomac.2017.01.021. Epub 2017 Jan 5.
Halophilic organic solvent tolerant protease (HOSP) producing Paracoccus saliphilus APCMST-CS5 was isolated from the marine sediment samples and identified through 16S rRNA sequence analysis. P. saliphilus APCMST-CS5 registered maximum HOSP production of 1,321.70U/ml in the medium contained the most significant parameters such as shrimp shell powder (SSP), CaCl, NaCl, and sardinella powder (SP), obtained through Placket-Burman and Response Surface Methods. HOSP was further purified to 22.68 fold purity with 29.71 U/mg specific activity and its molecular weight was 39kDa. The HOSP was stable at 60°C, 9.0 pH, 3.0M NaCl concentration and it also showed maximum activity at other tested parameters. Interestingly the purified HOSP showed better antibiofilm ability against tested pathogens. Also, the HOSP effectively deproteinized (85.64%) shrimp shell chitin which in turn maximum and exhibited higher antioxidant activity. The commercial and experimental shrimp shell chitin showed similar peak pattern in FTIR and C CP/MAS NMR spectral analysis.
从海洋沉积物样本中分离出了产嗜盐有机溶剂耐受蛋白酶（HOSP）的嗜盐副球菌APCMST-CS5，并通过16S rRNA序列分析进行了鉴定。嗜盐副球菌APCMST-CS5在含有虾壳粉（SSP）、氯化钙、氯化钠和沙丁鱼粉（SP）等最显著参数的培养基中，HOSP产量最高可达1321.70U/ml，这些参数是通过Placket-Burman法和响应面法获得的。HOSP进一步纯化至22.68倍纯度，比活性为29.71U/mg，其分子量为39kDa。该HOSP在60°C、pH 9.0、3.0M氯化钠浓度下稳定，在其他测试参数下也表现出最大活性。有趣的是，纯化后的HOSP对测试病原体表现出更好的抗生物膜能力。此外，HOSP能有效去除虾壳几丁质中的蛋白质（85.64%），进而使虾壳几丁质达到最大量并表现出更高的抗氧化活性。在傅里叶变换红外光谱（FTIR）和交叉极化/魔角旋转核磁共振光谱（C CP/MAS NMR）分析中，商业虾壳几丁质和实验虾壳几丁质显示出相似的峰型。
