Maruthiah Thirumalai, Somanath Beena, Jasmin Jebamonydhas Vijila, Immanuel Grasian, Palavesam Arunachalam
Centre for Marine Science and Technology, Manonmaniam Sundaranar University, Rajakkamangalam, Kanyakumari, 629 502, Tamilnadu, India.
Department of Zoology, Rani Anna Government College for Women, Manonmaniam Sundaranar University, Tirunelveli, 627 012, Tamilnadu, India.
3 Biotech. 2016 Dec;6(2):157. doi: 10.1007/s13205-016-0474-y. Epub 2016 Jul 26.
The quantum of marine fish wastes produced by fish processing industries has necessitated to search new methods for its disposal. Hence, this study is focused on production and purification of halophilic organic solvent tolerant protease (HOSP) from marine Alcaligenes faecalis APCMST-MKW6 using marine shell wastes as substrate. The candidate bacterium was isolated from the marine sediment of Manakudi coast and identified as A. faecalis APCMST-MKW6. The purified protease showed 16.39-fold purity, 70.34 U/mg specific activity with 21.67 % yield. The molecular weight of the purified alkaline protease was 49 kDa. This purified protease registered maximum activity at pH 9 and it was stable between pH 8-9 after 1.30 h of incubation. The optimum temperature registered was 60 °C and it was stable between 50 and 60 °C even after 1.30 h of incubation. This enzyme also showed maximum activity at 20 % NaCl concentration. Further, manganese chloride, magnesium chloride, calcium chloride and barium chloride influenced this enzyme activity remarkably and it was also found to be enhanced by many of the tested surfactants and solvents. The candidate bacterium effectively deproteinized the shrimp shell waste compared to the other tested crustaceans shell wastes and also attained maximum antioxidant activity.
鱼类加工行业产生的海洋鱼类废弃物数量巨大，因此有必要寻找新的处理方法。因此，本研究聚焦于以海洋贝壳废弃物为底物，从海洋粪产碱菌APCMST-MKW6中生产和纯化嗜盐耐有机溶剂蛋白酶（HOSP）。候选细菌从马纳库迪海岸的海洋沉积物中分离出来，并鉴定为粪产碱菌APCMST-MKW6。纯化后的蛋白酶纯度提高了16.39倍，比活性为70.34 U/mg，产率为21.67%。纯化后的碱性蛋白酶分子量为49 kDa。该纯化蛋白酶在pH 9时活性最高，孵育1.30小时后在pH 8 - 9之间保持稳定。最佳温度为60°C，孵育1.30小时后在50至60°C之间仍保持稳定。该酶在20% NaCl浓度下也表现出最高活性。此外，氯化锰、氯化镁、氯化钙和氯化钡对该酶活性有显著影响，并且还发现许多测试的表面活性剂和溶剂能增强其活性。与其他测试的甲壳类动物壳废弃物相比，候选细菌能有效地使虾壳废弃物脱蛋白，并且还具有最大的抗氧化活性。
