Tumor Etiology and Screening Department of Cancer Institute and General Surgery, the First Affiliated Hospital of China Medical University, and Key Laboratory of Cancer Etiology and Prevention (China Medical University), Liaoning Provincial Education Department, Shenyang 110001, People's Republic of China.
Central Laboratory, Cancer Hospital of China Medical University, Liaoning Cancer Hospital &Institute, Shenyang 110042, People's Republic of China.
Sci Rep. 2017 Jan 9;7:39528. doi: 10.1038/srep39528.
We aimed to explore the associations of polymorphisms in three microRNAs (miRNAs) (let-7e rs8111742, miR-365b rs121224 and miR-4795 rs1002765) that target PGC with the risk and prognosis of gastric cancer/atrophic gastritis. Sequenom's MassArray was used to genotype the miRNA polymorphisms in 724 gastric cancer cases, 862 atrophic gastritis cases and 862 controls in a Chinese population. We found that let-7e rs8111742 and miR-4795 rs1002765 were associated with the risk of gastric cancer in the H. pylori-positive subgroup. MiR-365b rs121224 was associated with the risk of intestinal-type gastric cancer in the alcohol consumption subgroup. Intestinal-type gastric cancer patients at Borrmann stages III-IV who carry the miR-365b rs121224 GG genotype had better prognosis compared with those who carry the CG or CC genotypes. MiR-365b rs121224 was associated with Lauren typing and TNM staging, in which the distribution of GG genotype carriers in intestinal-type gastric cancer and the TNM stage I-II subgroup was higher than that of CG or CC genotypes, which contrasted with the distribution in diffuse-type gastric cancer or TNM III-IV groups. These findings suggested that the polymorphisms in these miRNAs might be biomarkers for gastric cancer risk and prognosis, especially for populations infected with Helicobacter pylori or who consume alcohol.
我们旨在探讨三个 microRNA（miRNA）（let-7e rs8111742、miR-365b rs121224 和 miR-4795 rs1002765）的多态性与靶向 PGC 的 miRNA 与胃癌/萎缩性胃炎风险和预后的关联。在中国人群中，我们使用 Sequenom 的 MassArray 对 724 例胃癌病例、862 例萎缩性胃炎病例和 862 例对照中的 miRNA 多态性进行了基因分型。我们发现，let-7e rs8111742 和 miR-4795 rs1002765 与 H. pylori 阳性亚组的胃癌风险相关。miR-365b rs121224 与酒精消费亚组的肠型胃癌风险相关。Borrmann 分期为 III-IV 期的肠型胃癌患者携带 miR-365b rs121224 GG 基因型的预后优于携带 CG 或 CC 基因型的患者。miR-365b rs121224 与 Lauren 分型和 TNM 分期相关，其中肠型胃癌和 TNM Ⅰ-Ⅱ亚组 GG 基因型携带者的分布高于 CG 或 CC 基因型，这与弥漫型胃癌或 TNM Ⅲ-Ⅳ 组的分布形成对比。这些发现表明，这些 miRNA 的多态性可能是胃癌风险和预后的生物标志物，特别是对感染幽门螺杆菌或饮酒的人群。
