Weng Yin-Hua, Yu Wen-Tao, Luo Yan-Ping, Liu Chao, Gu Xi-Xi, Chen Huo-Ying, Liu Hong-Bo
Department of Laboratory Medicine, The Second Affiliated Hospital of Guilin Medical University, Guilin, China.
College of Medical Laboratory Science, Guilin Medical University, Guilin, China.
Front Neurol. 2023 Sep 27;14:1260230. doi: 10.3389/fneur.2023.1260230. eCollection 2023.
Ischemic stroke (IS) represents a major cause of morbidity and mortality across the globe. The aberrant expression of miR-365 has been found to be implicated in a wide array of human diseases, including atherosclerosis and cancer. Studies on single-nucleotide polymorphisms (SNPs) in miRNA genes can help gain insight into the susceptibility to the condition. This study aimed to examine the relationship between miR-365 SNPs and the risk of IS.
The study recruited 215 IS patients and 220 controls. The SNPscans genotyping was employed to genotype three polymorphic loci (rs121224, rs30230, and rs178553) of miR-365. The relative expression of miR-365 in peripheral blood mononuclear cells of the patients and controls was determined by using real-time quantitative PCR.
The miR-365 rs30230 polymorphism exhibited a significant association with the risk of developing IS (TC vs. CC: adjusted OR = 0.55, 95% CI: 0.33-0.92, = 0.022; TT vs. CC: adjusted OR = 0.34, 95% CI: 0.14-0.85, = 0.021; TC +TT vs. CC: adjusted OR = 0.51, 95% CI: 0.31-0.83, = 0.007; T vs. C: adjusted OR = 0.57, 95% CI: 0.39-0.83, = 0.004). Haplotype analysis revealed that the C-T-G haplotype was associated with a decreased risk of IS (OR = 0.68, 95% CI: 0.46-1.00, = 0.047). Furthermore, miR-365 expression was significantly higher in IS patients than in controls ( < 0.001). Interestingly, patients with rs30230 TC or TT genotypes had lower miR-365 levels compared to their counterparts with CC genotypes ( < 0.001).
The miR-365 rs30230 polymorphism might bear an association with IS susceptibility in the Chinese population, and the rs30230 TC/TT genotype might be a protective factor against IS.
缺血性中风（IS）是全球发病和死亡的主要原因。已发现miR - 365的异常表达与包括动脉粥样硬化和癌症在内的多种人类疾病有关。对miRNA基因中的单核苷酸多态性（SNP）进行研究有助于深入了解该病的易感性。本研究旨在探讨miR - 365 SNP与IS风险之间的关系。
本研究招募了215例IS患者和220例对照。采用SNPscans基因分型技术对miR - 365的三个多态性位点（rs121224、rs30230和rs178553）进行基因分型。通过实时定量PCR测定患者和对照外周血单个核细胞中miR - 365的相对表达。
miR - 365 rs30230多态性与发生IS的风险显著相关（TC与CC比较：校正OR = 0.55，95% CI：0.33 - 0.92，P = 0.022；TT与CC比较：校正OR = 0.34，95% CI：0.14 - 0.85，P = 0.021；TC + TT与CC比较：校正OR = 0.51，95% CI：0.31 - 0.83，P = 0.007；T与C比较：校正OR = 0.57，95% CI：0.39 - 0.83，P = 0.004）。单倍型分析显示，C - T - G单倍型与IS风险降低相关（OR = 0.68，95% CI：0.46 - 1.00，P = 0.047）。此外，IS患者中miR - 365表达显著高于对照组（P < 0.001）。有趣的是，与CC基因型的患者相比，rs30230 TC或TT基因型的患者miR - 365水平较低（P < 0.001）。
miR - 365 rs30230多态性可能与中国人群的IS易感性相关，rs30230 TC/TT基因型可能是IS的保护因素。
