AK Project, Graduate School of Medicine, Kyoto University, Kyoto 606-8501, Japan.
Division of Biomedical Science, Research School of Biology, The Australian National University, Canberra 0200, Australia.
Sci Rep. 2017 Jan 9;7:40401. doi: 10.1038/srep40401.
Development of lymphoid tissue is determined by interactions between stromal lymphoid tissue organiser (LTo) and hematopoietic lymphoid tissue inducer (LTi) cells. A failure for LTo to receive appropriate activating signals during embryogenesis through lymphotoxin engagement leads to a complete cessation of lymph node (LN) and Peyer's patch development, identifying LTo as a key stromal population for lymphoid tissue organogenesis. However, little is known about the equivalent stromal cells that induce spleen development. Here, by dissociating neonatal murine spleen stromal tissue for re-aggregation and transplant into adult mouse recipients, we have identified a MAdCAM-1CD31CD201 spleen stromal organizer cell-type critical for new tissue formation. This finding provides an insight into the regulation of post-natal spleen tissue organogenesis, and could be exploited in the development of spleen regenerative therapies.
淋巴组织的发育取决于基质淋巴组织组织者（LTo）和造血淋巴组织诱导细胞（LTi）之间的相互作用。在胚胎发生过程中，淋巴毒素结合导致 LTo 未能接收到适当的激活信号，从而导致淋巴结（LN）和派尔氏斑发育完全停止，这表明 LTo 是淋巴组织发生的关键基质细胞群。然而，对于诱导脾脏发育的等效基质细胞知之甚少。在这里，通过分离新生鼠脾脏基质组织进行重新聚集并移植到成年小鼠受体中，我们鉴定出一种 MAdCAM-1CD31CD201 关键的用于新组织形成的脾脏基质组织者细胞类型。这一发现为研究出生后脾脏组织发生的调控机制提供了思路，并可能为脾脏再生治疗的发展提供帮助。
