Clem Jones Research Centre for Regenerative Medicine, Bond University, Gold Coast, Queensland, Australia.
Research School of Biology, Australian National University, Canberra, Australian Capital Territory, Australia.
PLoS One. 2019 Oct 4;14(10):e0223416. doi: 10.1371/journal.pone.0223416. eCollection 2019.
Spleen stromal lines which support in vitro hematopoiesis are investigated for their lineage origin and hematopoietic support function in vivo. Marker expression and gene profiling identify a lineage relationship with mesenchymal stem cells and perivascular reticular cells described recently in bone marrow. Stromal lines commonly express Cxcl12, Pdgfra and Pdgfr typical of bone marrow derived perivascular reticular cells but reflect a unique cell type in terms of other gene and marker expression. Their classification as osteoprogenitors is confirmed through ability to undergo osteogenic, but not adipogenic or chondrogenic differentiation. Some stromal lines were shown to form ectopic niches for HSCs following engraftment under the kidney capsule of NOD/SCID mice. The presence of myeloid cells and a higher representation of a specific dendritic-like cell type over other myeloid cells within grafts was consistent with previous in vitro evidence of hematopoietic support capacity. These studies reinforce the role of perivascular/perisinusoidal reticular cells in hematopoiesis and implicate such cells as niches for hematopoiesis in spleen.
正在研究支持体外造血的脾基质细胞系，以研究其在体内的谱系起源和造血支持功能。标志物表达和基因谱分析确定了与骨髓中最近描述的间充质干细胞和血管周网状细胞的谱系关系。基质细胞系通常表达 Cxcl12、Pdgfra 和典型的骨髓来源的血管周网状细胞的 Pdgfr，但就其他基因和标志物表达而言，它们反映了一种独特的细胞类型。通过能够进行成骨分化，但不能进行成脂或软骨分化，证实了它们作为成骨前体细胞的分类。一些基质细胞系在 NOD/SCID 小鼠肾包膜下植入后，被证明可以为 HSCs 形成异位龛。移植物中髓系细胞的存在以及特定树突状样细胞类型的更高代表性超过其他髓系细胞，与之前体外造血支持能力的证据一致。这些研究加强了血管周/窦周网状细胞在造血中的作用，并暗示这些细胞是脾造血的龛位。
