Fu Aikun, Wang Yang, Wu Yanping, Chen Hongliang, Zheng Shasha, Li Yali, Xu Xin, Li Weifen
Key Laboratory of Animal Molecular Nutrition of Education of Ministry, College of Animal Sciences, Zhejiang University, Hangzhou, 310058, China.
Key Laboratory of Animal Feed and Nutrition of Zhejiang Province, Hangzhou, 310058, China.
J Cell Biochem. 2017 Sep;118(9):2664-2671. doi: 10.1002/jcb.25875. Epub 2017 May 15.
Echinacea purpurea is an indigenous North American purple cone flower used by North Americans for treatment of various infectious diseases and wounds. This study investigated the effect of polysaccharide enriched extract of Echinacea purpurea (EE) on the polarization of macrophages. The results showed that 100 µg/mL of EE could markedly activate the macrophage by increasing the expression of CD80, CD86, and MHCII molecules. Meanwhile, EE upregulated the markers of classically activated macrophages (M1) such as CCR7 and the production of IL-1β, IL-6, IL-12p70, TNF-αand NO. The functional tests showed that EE enhanced the phagocytic and intracellular bactericidal activity of macrophage against ST. Furthermore, we demonstrated that JNK are required for EE-induced NO and M1-related cytokines production. Together, these results demonstrated that EE can polarize macrophages towards M1 phenotype, which is dependent on the JNK signaling pathways. J. Cell. Biochem. 118: 2664-2671, 2017. © 2017 Wiley Periodicals, Inc.
紫锥菊是一种原产于北美的紫色松果菊，北美居民用它来治疗各种传染病和伤口。本研究调查了紫锥菊多糖富集提取物（EE）对巨噬细胞极化的影响。结果显示，100μg/mL的EE可通过增加CD80、CD86和MHCII分子的表达来显著激活巨噬细胞。同时，EE上调了经典激活巨噬细胞（M1）的标志物，如CCR7以及IL-1β、IL-6、IL-12p70、TNF-α和NO的产生。功能测试表明，EE增强了巨噬细胞对ST的吞噬和细胞内杀菌活性。此外，我们证明JNK是EE诱导NO和M1相关细胞因子产生所必需的。总之，这些结果表明EE可使巨噬细胞向M1表型极化，这依赖于JNK信号通路。《细胞生物化学杂志》118: 2664 - 2671, 2017年。© 2017威利期刊公司
