Department of Gastroenterology, The First Hospital of Jilin University , Changchun 130021, China.
State Key Laboratory on Integrated Optoelectronics, College of Electronic Science and Engineering, Jilin University , Changchun 130012, China.
ACS Appl Mater Interfaces. 2017 Feb 1;9(4):3419-3431. doi: 10.1021/acsami.6b14325. Epub 2017 Jan 18.
Photodynamic therapy (PDT) is a promising treatment modality for clinical cancer therapy. However, the therapeutic effect of PDT is strongly dependent on the property of photosensitizer. Here, we developed photo-cross-linkable semiconductor polymer dots doped with photosensitizer Chlorin e6 (Ce6) to construct a nanoparticle platform for photodynamic therapy. Photoreactive oxetane groups were attached to the side chains of the semiconductor polymer. After photo-cross-linking reaction, the Ce6-doped Pdots formed an interpenetrated structure to prevent Ce6 leaching out from the Pdot matrix. Spectroscopic characterizations revealed an efficient energy transfer from the polymer to Ce6 molecules, resulting in amplified generation of singlet oxygen. We evaluated the cellular uptake, cytotoxicity, and photodynamic effect of the Pdots in gastric adenocarcinoma cells. In vitro photodynamic experiments indicated that the Ce6-doped Pdots (∼10 μg/mL) effectively killed the cancer cells under low dose of light irradiation (∼60 J/cm). Furthermore, in vivo photodynamic experiments were carried out in tumor-bearing nude mice, which indicated that the Pdot photosensitizer apparently suppressed the growth of solid tumors. Our results demonstrate that the photo-cross-linkable Pdots doped with photosensitizer are promising for photodynamic cancer treatment.
光动力疗法（PDT）是一种很有前途的临床癌症治疗方法。然而，PDT 的治疗效果强烈依赖于光敏剂的性质。在这里，我们开发了一种掺杂光敏剂氯己定（Ce6）的可光交联半导体聚合物点，构建了用于光动力治疗的纳米颗粒平台。光反应性氧杂环丁烷基团被连接到半导体聚合物的侧链上。光交联反应后，Ce6 掺杂的 Pdots 形成互穿结构，以防止 Ce6 从 Pdot 基质中浸出。光谱特性研究表明，聚合物有效地将能量转移到 Ce6 分子上，从而放大了单线态氧的生成。我们评估了 Pdots 在胃腺癌细胞中的细胞摄取、细胞毒性和光动力效应。体外光动力实验表明，Ce6 掺杂的 Pdots（约 10μg/mL）在低剂量光照射（约 60J/cm）下有效地杀死了癌细胞。此外，在荷瘤裸鼠体内进行了光动力实验，结果表明 Pdot 光敏剂明显抑制了实体瘤的生长。我们的结果表明，掺杂光敏剂的可光交联 Pdots 有望用于光动力癌症治疗。
