Pearce Kaycey, Cai Diancai, Roberts Adam C, Glanzman David L
Department of Integrative Biology and Physiology, UCLA, Los Angeles, United States.
Department of Neurobiology, David Geffen School of Medicine at UCLA, Los Angeles, United States.
Elife. 2017 Jan 9;6:e18299. doi: 10.7554/eLife.18299.
Previously, we reported that long-term memory (LTM) in can be reinstated by truncated (partial) training following its disruption by reconsolidation blockade and inhibition of PKM (Chen et al., 2014). Here, we report that LTM can be induced by partial training after disruption of original consolidation by protein synthesis inhibition (PSI) begun shortly after training. But when PSI occurs during training, partial training cannot subsequently establish LTM. Furthermore, we find that inhibition of DNA methyltransferase (DNMT), whether during training or shortly afterwards, blocks consolidation of LTM and prevents its subsequent induction by truncated training; moreover, later inhibition of DNMT eliminates consolidated LTM. Thus, the consolidation of LTM depends on two functionally distinct phases of protein synthesis: an early phase that appears to prime LTM; and a later phase whose successful completion is necessary for the normal expression of LTM. Both the consolidation and maintenance of LTM depend on DNA methylation.
此前，我们报道过，在长期记忆（LTM）被重新巩固阻断和PKM抑制破坏后，通过截断（部分）训练可以恢复（Chen等人，2014年）。在此，我们报告，在训练后不久开始通过蛋白质合成抑制（PSI）破坏原始巩固后，部分训练可以诱导LTM。但是当PSI在训练期间发生时，部分训练随后无法建立LTM。此外，我们发现，无论是在训练期间还是之后不久，抑制DNA甲基转移酶（DNMT）都会阻断LTM的巩固，并阻止其随后通过截断训练诱导产生；此外，后期抑制DNMT会消除已巩固的LTM。因此，LTM的巩固取决于蛋白质合成的两个功能不同的阶段：一个早期阶段，似乎为LTM做好准备；以及一个后期阶段，其成功完成对于LTM的正常表达是必要的。LTM的巩固和维持都依赖于DNA甲基化。
