Chen He, Song Yajuan, Liu Nina, Wan Hongchang, Shu Guowei, Liao Na
College of Life Science and Engineering, Shaanxi University of Science and Technology, China.
Shaanxi Yatai Dairy Co., Ltd. Xianyang, China.
Acta Sci Pol Technol Aliment. 2015 Jul-Sep;14(3):207-213. doi: 10.17306/J.AFS.2015.3.22.
Lactobacillus acidophilus has become increasingly popular because of their beneficial effects on health of their host, and are called proboscis. In order to exert beneficial effects for probiotics, they must be able to tolerate the acidic conditions of the stomach environment and the bile in the small intestine. Microencapsulated form has received reasonable attention, since it can protect probiotic organisms against an unfavourable environment, and to allow their release in a viable and metabolically active state in the intestine. The aim of this study was to investigate some factores, such as chitosan solution pH and concentration, xanthan concentration, cell suspension-xanthan ratio, mixed bacteria glue liquid-chitosan ratio, which impacted the process of microencapsulation of L. acidophilus.
In this study, L. acidophilus was immobilized with xanthan⁄chitosan gel using extrusion method. The viable counts and encapsulation yield of L. acidophilus encapsulated in different chitosan solution pH (4.5, 5, 5.5 and 6), in different chitosan concentration (0.5%, 0.7%, 0.9% and 1.1%), in different xanthan concentration (0.5%, 0.7%, 0.9% and 1.1%), in different cell suspension-xanthan ratios (1:5, 1:10, 1:15 and 1:20), in different mixed bacteria glue liquid-chitosan ratios (1:3, 1:4, 1:5 and 1:6), have been investigated by single factor experiment method.
The optimum conditions of microencapsulated L. acidophilus have been observed. The optimum chitosan solution pH for L. acidophilus was 5.5; the optimum chitosan concentration was 0.9%; the optimum xanthan concentration was 0.7%; the optimum cell suspension-xanthan ratio was 1:10; the optimum mixed bacteria glue liquid-chitosan ratio was 1:3.
These results will be helpful to further optimize the process of L. acidophilus microencapsulation, and provide reference for obtaining higher viable counts and entrapped yield of L. acidophilus microcapsules.
嗜酸乳杆菌因其对宿主健康的有益作用而越来越受欢迎，被称为益生菌。为了发挥益生菌的有益作用，它们必须能够耐受胃环境的酸性条件和小肠中的胆汁。微胶囊形式受到了合理关注，因为它可以保护益生菌免受不利环境的影响，并使其在肠道中以存活且代谢活跃的状态释放。本研究的目的是研究一些影响嗜酸乳杆菌微胶囊化过程的因素，如壳聚糖溶液的pH值和浓度、黄原胶浓度、细胞悬浮液与黄原胶的比例、混合菌胶液与壳聚糖的比例。
在本研究中，采用挤压法用黄原胶/壳聚糖凝胶固定嗜酸乳杆菌。通过单因素实验法研究了嗜酸乳杆菌在不同壳聚糖溶液pH值（4.5、5、5.5和6）、不同壳聚糖浓度（0.5%、0.7%、0.9%和1.1%）、不同黄原胶浓度（0.5%、0.7%、0.9%和1.1%）、不同细胞悬浮液与黄原胶比例（1:5、1:10、1:15和1:20）、不同混合菌胶液与壳聚糖比例（1:3、1:4、1:5和1:6）下的活菌数和包封率。
观察到嗜酸乳杆菌微胶囊化的最佳条件。嗜酸乳杆菌的最佳壳聚糖溶液pH值为5.5；最佳壳聚糖浓度为0.9%；最佳黄原胶浓度为0.7%；最佳细胞悬浮液与黄原胶比例为1:10；最佳混合菌胶液与壳聚糖比例为1:3。
这些结果将有助于进一步优化嗜酸乳杆菌微胶囊化工艺，并为获得更高活菌数和包封率的嗜酸乳杆菌微胶囊提供参考。
