双歧杆菌 BB01 的黄原胶-壳聚糖微胶囊化：制备及其在纯牛奶中的稳定性。
Microencapsulation of Bifidobacterium bifidum BB01 by xanthan-chitosan: preparation and its stability in pure milk.
机构信息
a School of Food and Biological Engineering , Shaanxi University of Science and Technology , Xi'an , PR China.
b College of Food Engineering and Nutritional Science , Shaanxi Normal University , Xi'an , PR China.
出版信息
Artif Cells Nanomed Biotechnol. 2018;46(sup1):588-596. doi: 10.1080/21691401.2018.1431652. Epub 2018 Jan 29.
Xanthan-chitosan (XC) and xanthan-chitosan-xanthan (XCX) were employed for microencapsulation of Bifidobacterium bifidum BB01 using extrusion technique. To optimize the process of B. bifidum BB01 microcapsules based on XC hydrogels, response surface methodology was employed to obtain the best possible combination of chitosan concentration, xanthan-B. bifidum BB01 mixture (XBM)/chitosan, stirring time for the maximum viable count and encapsulation yield. The optimum conditions were: chitosan concentration of 0.84 g·mL, XBM/chitosan of 1:9.0, stirring time of 60 min with high viable count and encapsulation yield of 1.52 ± 0.15 × 10CFU·g, 90 ± 0.65%, respectively. In addition, the effective encapsulation system (XC and XCX) resulted in improvement in survival of B. bifidum BB01 compared to non-encapsulated cells during 3 weeks storage at 4 and 25 °C in pure milk.
采用挤出技术，使用黄原胶-壳聚糖（XC）和黄原胶-壳聚糖-黄原胶（XCX）对双歧杆菌 BB01 进行微胶囊化。为了优化基于 XC 水凝胶的双歧杆菌 BB01 微胶囊的工艺，采用响应面法获得了最大活菌数和包埋率的最佳壳聚糖浓度、黄原胶-双歧杆菌 BB01 混合物（XBM）/壳聚糖、搅拌时间的最佳组合。最佳条件为：壳聚糖浓度为 0.84 g·mL-1，XBM/壳聚糖为 1:9.0，搅拌时间为 60 min，活菌数和包埋率分别为 1.52±0.15×10CFU·g-1、90±0.65%。此外，与未包埋的细胞相比，有效的包埋系统（XC 和 XCX）在 4 和 25°C下在纯牛奶中储存 3 周时，提高了双歧杆菌 BB01 的存活率。
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