College of Pharmaceutical Sciences, Zhejiang University , 866 Yuhangtang Road, Hangzhou 310058, People's Republic of China.
Key Laboratory of Precision Diagnosis and Treatment for Hepatobiliary and Pancreatic Tumor of Zhejiang Province, The First Affiliated Hospital, College of Medicine, Zhejiang University , 79 QingChun Road, Hangzhou 310000, People's Republic of China.
ACS Appl Mater Interfaces. 2017 Feb 1;9(4):3354-3367. doi: 10.1021/acsami.6b13351. Epub 2017 Jan 20.
Both photothermal therapy (PTT) and photodynamic therapy (PDT) are phototherapeutic approaches, which have been widely investigated for cancer therapy mediated by an external light source. Here, a nanosystem presenting the synchronous PTT and PDT effect realized through one-step near-infrared (NIR) light irradiation is reported. This system was fabricated by conjugating indocyanine green (ICG) on hollow gold nanospheres (HAuNS) using branched-polyethylenimine (PEI, MW = 10 kDa) as optimal linker, which provided a high ICG payload as well as a covering layer with suitable thickness on HAuNS to maintain ICG fluorescence and reactive oxygen species (ROS) productivity. The resulting system (ICG-PEI-HAuNS) had the molar ratio of ICG:PEI:Au = 3:0.33:5. Compared with free ICG, ICG-PEI-HAuNS exhibited dramatically enhanced stability of ICG molecules and greater intratumoral accumulation. The conjugation of ICG caused significantly higher plasmon absorption of ICG-PEI-HAuNS in the NIR region compared with HAuNS alone, inducing remarkably enhanced photothermal conversion efficiency and synchronous photodynamic effect under NIR light irradiation. Interestingly, compared with PTT or PDT alone, synchronous PTT and PDT produced by ICG-PEI-HAuNS upon NIR light irradiation induced significantly stronger antitumor and metastasis inhibition effects both in vitro and in vivo, which might be a promising strategy for cancer treatment.
光热疗法（PTT）和光动力疗法（PDT）都是光疗方法，已经广泛研究用于通过外部光源介导的癌症治疗。在这里，报道了一种通过一步近红外（NIR）光照射呈现同步 PTT 和 PDT 效应的纳米系统。该系统通过使用支化聚乙烯亚胺（PEI，MW = 10 kDa）作为最佳连接体将吲哚菁绿（ICG）共轭到中空金纳米球（HAuNS）上来制备，该连接体提供了高 ICG 有效负载以及具有合适厚度的覆盖层，以维持 HAuNS 上的 ICG 荧光和活性氧（ROS）产生能力。所得系统（ICG-PEI-HAuNS）的 ICG:PEI:Au 的摩尔比为 3:0.33:5。与游离 ICG 相比，ICG-PEI-HAuNS 表现出 ICG 分子显著增强的稳定性和更大的肿瘤内积累。与单独的 HAuNS 相比，ICG 的共轭导致 ICG-PEI-HAuNS 在 NIR 区域具有明显更高的等离子体吸收，在 NIR 光照射下诱导明显增强的光热转换效率和同步光动力效应。有趣的是，与单独的 PTT 或 PDT 相比，ICG-PEI-HAuNS 在 NIR 光照射下产生的同步 PTT 和 PDT 在体外和体内均引起了更强的抗肿瘤和转移抑制作用，这可能是癌症治疗的一种有前途的策略。
