Cretella Gilda, Lajolo Carlo, Castagnola Raffaella, Somma Francesco, Inchingolo MariaTeresa, Marigo Luca
1 Department of Endodontics, School of Dentistry, Catholic University of Sacred Heart , Rome, Italy .
2 Department of Oral Pathology, School of Dentistry, Catholic University of Sacred Heart , Rome, Italy .
Photomed Laser Surg. 2017 Apr;35(4):190-194. doi: 10.1089/pho.2016.4174. Epub 2017 Jan 9.
This study examined the bactericidal effect of diode laser irradiation against intracanal Enterococcus faecalis.
m total of 128 extracted single-rooted and single-canal teeth were treated with ProTaper instruments (Dentsply Maillefer, Ballaigues, Switzerland). A total of 120 root canals were inoculated with E. faecalis for 21 days, and the samples were randomly divided into five groups: Group 1 (n = 24) samples were irrigated with only saline solution (positive controls); Group 2 (n = 24) was treated with only 5.25% sodium hypochlorite; Group 3 (n = 24) was irrigated with saline solutions activated by diode laser; Group 4 (n = 24) was treated with 5.25% sodium hypochlorite activated by diode laser; and Group 5 (n = 24) was irrigated with saline solution with methylene blue dye activated by the diode laser Fox (Sweden & Martina, Padova, Italy); additionally, eight teeth were not contaminated and their canals were irrigated with saline solution and used as a negative control. The Uro-Quick system was used to determine the microbial residual charge. The data were analyzed using Pearson's chi-square test (p < 0.001).
A statistically significant reduction in bacterial count was observed in Group 2 and Group 4 (p < 0.001). There were no statistically significant differences among the other groups (p > 0.001).
Evidence indicates that the diode laser was not more effective than sodium hypochlorite in reducing free bacteria.
本研究检测二极管激光照射对根管内粪肠球菌的杀菌效果。
总共128颗单根单管的离体牙用ProTaper器械（登士柏迈弗，瑞士巴拉格）进行处理。总共120个根管接种粪肠球菌21天，样本随机分为五组：第1组（n = 24）样本仅用盐溶液冲洗（阳性对照）；第2组（n = 24）仅用5.25%次氯酸钠处理；第3组（n = 24）用二极管激光激活的盐溶液冲洗；第4组（n = 24）用二极管激光激活的5.25%次氯酸钠处理；第5组（n = 24）用二极管激光激活的含亚甲蓝染料的盐溶液冲洗（瑞典&玛蒂娜，意大利帕多瓦）；另外，8颗未受污染的牙齿，其根管用盐溶液冲洗并用作阴性对照。使用Uro-Quick系统测定微生物残留量。数据采用Pearson卡方检验进行分析（p < 0.001）。
第2组和第4组细菌计数有统计学显著降低（p < 0.001）。其他组之间无统计学显著差异（p > 0.001）。
有证据表明，在减少游离细菌方面，二极管激光并不比次氯酸钠更有效。
