Lobato Marcelo Figueiredo, Turssi Cecilia Pedroso, Amaral Flávia Lucisano Botelho do, França Fabiana Mantovani Gomes, Basting Roberta Tarkany
Gen Dent. 2017 Jan-Feb;65(1):62-66.
The aim of this study was to investigate the antimicrobial effect of an experimental adhesive system containing chitosan (0.2% and 0.5%) against Streptococcus mutans and Lactobacillus casei. Twenty-four extracted human third molars were used, and 4 cavities were prepared in each tooth. The teeth were contaminated with either S mutans (n = 12 teeth, 48 cavities) or L casei (n = 12 teeth, 48 cavities) microorganisms. One cavity in each tooth received 1 of the following treatments: negative control (no treatment [NC]), positive control (Adper Single Bond 2 [SB]), an experimental adhesive containing chitosan 0.2% (CHI2), or an experimental adhesive containing chitosan 0.5% (CHI5). After sealing of the cavities and an incubation period, dentin scrapings were collected from each cavity for microbiological evaluation. Analysis of variance and Tukey tests revealed no statistically significant differences among the SB, CHI2, and CHI5 groups (P > 0.05), but all 3 differed significantly from the NC group (P ≤ 0.05). The concentrations of chitosan did not influence the antimicrobial effect against S mutans and L casei, presenting a similar effect to that of a conventional 2-step adhesive system.
本研究的目的是调查含壳聚糖（0.2%和0.5%）的实验性粘结系统对变形链球菌和干酪乳杆菌的抗菌效果。使用了24颗拔除的人类第三磨牙，每颗牙齿制备4个窝洞。这些牙齿分别被变形链球菌（n = 12颗牙齿，48个窝洞）或干酪乳杆菌（n = 12颗牙齿，48个窝洞）微生物污染。每颗牙齿的一个窝洞接受以下一种治疗：阴性对照（不治疗[NC]）、阳性对照（Adper Single Bond 2[SB]）、含0.2%壳聚糖的实验性粘结剂（CHI2）或含0.5%壳聚糖的实验性粘结剂（CHI5）。窝洞封闭并经过一段培养期后，从每个窝洞收集牙本质刮屑进行微生物学评估。方差分析和Tukey检验显示，SB、CHI2和CHI5组之间无统计学显著差异（P > 0.05），但这3组与NC组均有显著差异（P≤0.05）。壳聚糖浓度对变形链球菌和干酪乳杆菌的抗菌效果没有影响，其效果与传统的两步粘结系统相似。
