Faculty of Dentistry, Department of Restorative Dentistry, Altinbas University, Istanbul, Turkey.
Institute of Graduate Studies in Health Sciences, Istanbul University, Istanbul, Turkey.
BMC Oral Health. 2024 Oct 14;24(1):1220. doi: 10.1186/s12903-024-05001-2.
The objective of this study is to investigate the shear bonding strength of a glycoalkaloid, also a novel matrix metalloproteinase enzyme known as α-tomatine, on two different surfaces of dentin (sound & caries-affected) and its efficacy against cariogenic microorganisms using in vitro and in silico methods.
The effect of a-tomatine at different concentrations (0.75 / 1 / 1.5 µM) on shear bonding strength in caries-affected and sound dentin was also investigated (n = 10; each per subgroup). The analysis of shear bonding and failure tests was conducted after a 24-hour storage period. Fracture surfaces were examined under a scanning electron microscope. A stock solution 3 mM of a-tomatine was prepared for antimicrobial evaluation. Antimicrobial activities of the agents against Streptococcus mutans ATCC 25175, Lactobacillus casei ATCC 4646, and Candida albicans ATCC 10231 standard strains were investigated by microdilution method. In addition, through the method of molecular docking and dynamic analysis, the affinity of a-tomatine for certain enzymes of these microorganisms was examined.
The pretreatment agent and dentin type significantly influenced shear bonding strength values (p < 0.05). As the molarity of a-tomatine increased, the bonding value decreased in sound dentin, while the opposite was true in caries-affected dentin. According to molecular docking and dynamic analysis, the highest affinity was observed in L. casei's signaling protein. Microdilution assays revealed a-tomatine to exhibit fungicidal activity against C. albicans and bacteriostatic effects against S. mutans. No antimicrobial effect was observed on L. casei.
a-tomatine demonstrates a positive impact by serving as both a pretreatment agent for bonding strength and an inhibitor against certain cariogenic microorganisms.
本研究旨在通过体外和计算机模拟方法研究糖苷生物碱（即一种新型基质金属蛋白酶酶 α-茄碱）在两种不同牙本质表面（健康和龋损）上的剪切粘结强度及其对致龋微生物的作用。
还研究了不同浓度（0.75/1/1.5 µM）的α-茄碱对龋损和健康牙本质剪切粘结强度的影响（n = 10；每组各 10 个）。在 24 小时储存期后进行剪切粘结和失效测试分析。在扫描电子显微镜下检查断裂面。为了进行抗菌评估，制备了 3 mM 的 α-茄碱储备溶液。采用微量稀释法研究了该试剂对变异链球菌 ATCC 25175、干酪乳杆菌 ATCC 4646 和白假丝酵母 ATCC 10231 标准菌株的抗菌活性。此外，通过分子对接和动态分析方法，研究了 α-茄碱与这些微生物某些酶的亲和力。
预处理剂和牙本质类型显著影响剪切粘结强度值（p < 0.05）。随着 α-茄碱的摩尔浓度增加，在健康牙本质中粘结值降低，而在龋损牙本质中则相反。根据分子对接和动态分析，在干酪乳杆菌的信号蛋白中观察到最高的亲和力。微量稀释试验显示 α-茄碱对白色念珠菌具有杀菌活性，对变异链球菌具有抑菌作用。对干酪乳杆菌未观察到抗菌作用。
α-茄碱作为粘结强度的预处理剂和某些致龋微生物的抑制剂具有积极影响。
