School of Chemistry and Chemical Engineering, Shandong University , Jinan, Shandong 250100, China.
School of Food Science and Engineering, Qilu University of Technology , Jinan, Shandong 250353, China.
ACS Appl Mater Interfaces. 2017 Feb 1;9(4):3514-3523. doi: 10.1021/acsami.6b15637. Epub 2017 Jan 18.
Herein, we synthesized a cost-effective iron porphyrin (FePor)-based covalent organic polymer (COP), FePor-TFPA-COP, through an easy aromatic substitution reaction between pyrrole and tris(4-formylphenyl)amine (TFPA). The triangular pyramid-shaped, N-centric structure of TFPA facilitated the formation of FePor-TFPA-COP with three-dimensional porous structure, larger surface area, and abundant surface catalytically active sites. FePor-TFPA-COP exhibited strong intrinsic peroxidase activity toward a classical peroxidase substrate, 3,3',5,5'-tetramethylbenzidine (TMB), in the presence of HO. Compared with horseradish peroxidase (HRP), FePor-TFPA-COP exhibited several advantages such as easy storage, high sensitivity, and prominently chemical and catalytic stability under the harsh conditions, which guaranteed the accuracy and reliability of measurements. Utilizing the excellent catalytic activity, a FePor-TFPA-COP-based colorimetric immunoassay was first established for α-fetoprotein (AFP) detection and showed high sensitivity, stability, and acceptable reproducibility. The linear response range for AFP was 5 pg/mL to 100 ng/mL and the detection limitation was 1 pg/mL. The routine provided a brilliant biomimetic catalyst to develop the nonenzyme immunoassay. More importantly, the high chemical and catalytic stability and sensitivity facilitated future practical applications under various conditions.
在此，我们通过吡咯和三（4-醛基苯基）胺（TFPA）之间的简单芳香取代反应，合成了一种具有成本效益的铁卟啉（FePor）基共价有机聚合物（COP），FePor-TFPA-COP。TFPA 的三角锥形、N 中心结构促进了具有三维多孔结构、更大表面积和丰富表面催化活性位点的 FePor-TFPA-COP 的形成。在 HO 的存在下，FePor-TFPA-COP 对经典过氧化物酶底物 3,3',5,5'-四甲基联苯胺（TMB）表现出强烈的固有过氧化物酶活性。与辣根过氧化物酶（HRP）相比，FePor-TFPA-COP 具有几个优势，例如在恶劣条件下易于储存、高灵敏度以及明显的化学和催化稳定性，这保证了测量的准确性和可靠性。利用优异的催化活性，首次建立了基于 FePor-TFPA-COP 的比色免疫测定法用于检测α-胎蛋白（AFP），并表现出高灵敏度、稳定性和可接受的重现性。AFP 的线性响应范围为 5 pg/mL 至 100 ng/mL，检测限为 1 pg/mL。该常规方法为开发非酶免疫测定法提供了一种出色的仿生催化剂。更重要的是，高化学和催化稳定性和灵敏度为未来在各种条件下的实际应用提供了便利。
