Key Laboratory of Functional Small Organic Molecule, Ministry of Education, Key Laboratory of Chemical Biology, Jiangxi Province, College of Chemistry and Chemical Engineering, Jiangxi Normal University, Nanchang 330022, China.
Key Laboratory of Functional Small Organic Molecule, Ministry of Education, Key Laboratory of Chemical Biology, Jiangxi Province, College of Chemistry and Chemical Engineering, Jiangxi Normal University, Nanchang 330022, China.
Mater Sci Eng C Mater Biol Appl. 2020 Jul;112:110864. doi: 10.1016/j.msec.2020.110864. Epub 2020 Mar 20.
Here, a novel iron-porphyrin-based covalent organic framework (COF) was synthesized and applied for electrochemical sensing HO and pH which involved in many biological processes. The COF was obtained by post-modification of porphyrin-based COF (COF) which was firstly synthesized by aldehyde-ammonia condensation reaction between 1,3,5-benzenetricarboxaldehyde and 5,10,15,20-tetrakis(4-aminophenyl)-21H,23H- porphine. The COF was proved to be regular and uniform spherical particles with diameter about 1 μm, as well as possessed good crystalline structure and abundant micropores of about 1.4 nm. The resulted COF after post-modification with Fe maintained the original shape and crystalline structure of COF, while the micropores decreased to be about 0.89 nm. Electrochemical results indicated that the synthesized COF had good electrochemical redox and proton activity owing to iron-porphyrin, enabling to simultaneously be used as mimic peroxidase to catalyze the reduction of hydrogen peroxide (HO) and evaluate pH using current and potential as signal, respectively. The prepared sensor showed good performance for HO detection from 6.85 nM to 7 μM with the detection limit of 2.06 nM (S/N = 3), and pH test from 3.0 to 9.0. This work demonstrated that the iron-porphyrin-based COF could be used as a mimic peroxidase to apply in biological fields.
这里，合成了一种新型的基于铁卟啉的共价有机骨架（COF），并将其应用于电化学传感 HO 和 pH，这两者都涉及到许多生物过程。该 COF 是通过对卟啉基 COF（COF）进行后修饰得到的，COF 是通过 1,3,5-苯三甲醛和 5,10,15,20-四（4-氨基苯基）-21H,23H-卟啉之间的醛-氨缩合反应首次合成的。COF 被证明是具有约 1μm 直径的规则且均匀的球形颗粒，并且具有良好的结晶结构和丰富的约 1.4nm 的微孔。用 Fe 进行后修饰后得到的 COF 保持了 COF 的原始形状和结晶结构，而微孔减少至约 0.89nm。电化学结果表明，由于铁卟啉，合成的 COF 具有良好的电化学氧化还原和质子活性，使其能够同时用作模拟过氧化物酶来催化过氧化氢（HO）的还原，并分别使用电流和电势作为信号来评估 pH。所制备的传感器在 HO 检测方面表现出良好的性能，从 6.85nM 到 7μM，检测限为 2.06nM（S/N=3），并且可以进行 pH 测试，范围从 3.0 到 9.0。这项工作表明，基于铁卟啉的 COF 可用作模拟过氧化物酶，应用于生物领域。
