Department of Chemistry, University of Kurdistan, 66177-15175, Sanandaj, Iran.
Research Center for Nanotechnology, University of Kurdistan, 66177-15175, Sanandaj, Iran.
J Nanobiotechnology. 2018 Nov 20;16(1):93. doi: 10.1186/s12951-018-0421-7.
Given the great benefits of artificial enzymes, a simple approach is proposed via assembling of Ni with hemin for synthesis of Ni-hemin metal-organic-frameworks (Ni-hemin MOFs) mimic enzyme. The formation of the Ni-hemin MOFs was verified by scanning electron microscopy, Transmission electron microscopy, X-ray powder diffraction, X-ray photoelectron spectroscopy, Fourier transform infrared spectroscopy, Energy-dispersive X-ray spectroscopy and UV-vis absorption spectroscopy. This novel nanocomposite exhibited surprising peroxidase like activity monitored by catalytic oxidation of a typical peroxidase substrate, 3,3,5,5'-tetramethylbenzidine, in the presence of HO. By using folic acid conjugated MOF nanocomposite as a recognition element, we develop a colorimetric assay for the direct detection of cancer cells.
The proposed sensor presented high sensitivity and selectivity for the detection of human breast cancer cells (MCF-7) and Human Caucasian gastric adenocarcinoma. By measuring UV-vis absorbance response, a wide detection range from 50 to 10 cells/mL with a detection limit as low as 10 cells/mLwas reached for MCF-7 cells. We further discuss therapeutics efficiency of Ni-hemin MOFs in the presence of HO and ascorbic acid. Peroxidase-mimic Ni-hemin MOFs as reactive oxygen species which could damage MCF-7 cancer cells, however for normal cells (human embryonic kidney HEK 293 cells) killing effect was negligible.
Based on these behaviors, the developed method offers a fast, easy and cheap assay for the interest in future diagnostic and treatment application.
鉴于人工酶的巨大益处，通过组装 Ni 和血红素来合成 Ni-血红素金属有机骨架（Ni-血红素 MOFs）模拟酶，提出了一种简单的方法。通过扫描电子显微镜、透射电子显微镜、X 射线粉末衍射、X 射线光电子能谱、傅里叶变换红外光谱、能量色散 X 射线光谱和紫外可见吸收光谱验证了 Ni-血红素 MOFs 的形成。该新型纳米复合材料表现出令人惊讶的过氧化物酶样活性，通过在 HO 存在下催化氧化典型过氧化物酶底物 3,3,5,5'-四甲基联苯胺来监测。通过使用叶酸偶联的 MOF 纳米复合材料作为识别元件，我们开发了一种用于直接检测癌细胞的比色测定法。
所提出的传感器对人乳腺癌细胞（MCF-7）和人白种胃腺癌的检测具有高灵敏度和选择性。通过测量紫外可见吸收响应，达到了 50 至 10 个细胞/mL 的宽检测范围，检测限低至 10 个细胞/mL。我们进一步讨论了 HO 和抗坏血酸存在下 Ni-血红素 MOFs 的治疗效率。过氧化物酶模拟的 Ni-血红素 MOFs 作为活性氧，可以破坏 MCF-7 癌细胞，然而对正常细胞（人胚肾 HEK 293 细胞）的杀伤作用可以忽略不计。
基于这些行为，所开发的方法为未来的诊断和治疗应用提供了一种快速、简单和廉价的分析方法。
