粟酒裂殖酵母的减数分裂重组缺陷突变体。
Meiotic recombination-deficient mutants of Schizosaccharomyces pombe.
作者信息
Ponticelli A S, Smith G R
机构信息
Fred Hutchinson Cancer Research Center, Seattle, Washington 98104.
出版信息
Genetics. 1989 Sep;123(1):45-54. doi: 10.1093/genetics/123.1.45.
Abstract
A mutant screen employing the ade6-M26 recombination hotspot was developed and used to isolate Schizosaccharomyces pombe mutants deficient in meiotic recombination. Nine rec mutations were recessive, defining six complementation groups, and reduced ade6 meiotic recombination 3-fold to greater than or equal to 300-fold when homozygous. Three recessive rec mutations analyzed further also reduced meiotic intragenic recombination at ura4 on chromosome III and intergenic recombination between pro2 and arg3 on chromosome I. The observed non-co-ordinate reductions of the recombinant frequencies in the three test intervals suggest a degree of locus (or intragenic vs. intergenic) specificity of the corresponding rec+ gene products. None of the mutations specifically inactivated the ade6-M26 hotspot. Additional rec genes may be identified with these methods.
摘要
利用ade6-M26重组热点进行了突变体筛选，并用于分离粟酒裂殖酵母减数分裂重组缺陷型突变体。九个rec突变是隐性的，定义了六个互补群，纯合时将ade6减数分裂重组降低了3倍至大于或等于300倍。进一步分析的三个隐性rec突变也降低了III号染色体上ura4的减数分裂基因内重组以及I号染色体上pro2和arg3之间的基因间重组。在三个测试区间观察到的重组频率的非协同降低表明相应rec+基因产物存在一定程度的基因座（或基因内与基因间）特异性。没有一个突变能特异性地使ade6-M26热点失活。用这些方法可能会鉴定出其他rec基因。
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