Lammertyn Elise J, Vandermeulen Elly, Bellon Hannelore, Everaerts Stephanie, Verleden Stijn E, Van Den Eynde Kathleen, Bracke Ken R, Brusselle Guy G, Goeminne Pieter C, Verbeken Erik K, Vanaudenaerde Bart M, Dupont Lieven J
Laboratory of Respiratory Diseases, Department of Clinical and Experimental Medicine, University of Leuven, UZ Herestraat 49, Box 706, 3000, Leuven, Belgium.
Translational Cell and Tissue Research Unit, Department of Imaging and Pathology, University of Leuven, Leuven, Belgium.
Respir Res. 2017 Jan 10;18(1):10. doi: 10.1186/s12931-016-0489-2.
Cystic fibrosis (CF) lung disease is characterised by vigorous airway inflammation eventually resulting in severe lung damage. This study aimed to describe the diversity of the inflammatory pattern in end-stage CF lungs by evaluating and quantifying which components of the innate and adaptive immunity are involved, and by assessing whether this is gender-specific.
CF explant lung tissue (n = 20) collected at time of transplantation and control tissue (n = 22) was sectioned (9 μm) and stained for neutrophils, eosinophils, mast cells, dendritic cells, macrophages, CD4 T cells, cytotoxic T cells and B cells. Quantification with special attention for immune cell location was performed.
Neutrophils, mast cells, dendritic cells, macrophages, CD4 T and cytotoxic T cells were significantly increased in CF compared to controls and there was a disproportionate increase of neutrophils around the airways in CF. Large amounts of lymphoid follicles were found in the CF lung and they had a skewed B cell/T cell composition. Upon subdividing the CF patients into a male and female population, eosinophils, mast cells and CD4 T cells were increased specifically in CF females. In this subpopulation, lymphoid follicles had less B cells and more CD8 T cells.
These data demonstrate a diverse inflammatory response in the CF lung, reflected by an increase of both myeloid and lymphoid immune cells. Inflammation in the CF lung appeared to be gender-specific in our population, as the significant increase of eosinophils, mast cells and CD4 T cells was especially notable in the female subpopulation.
囊性纤维化（CF）肺部疾病的特征是气道炎症剧烈，最终导致严重的肺损伤。本研究旨在通过评估和量化先天性和适应性免疫的哪些成分参与其中，并评估这是否具有性别特异性，来描述终末期CF肺部炎症模式的多样性。
在移植时收集的CF外植肺组织（n = 20）和对照组织（n = 22）被切成薄片（9μm），并对中性粒细胞、嗜酸性粒细胞、肥大细胞、树突状细胞、巨噬细胞、CD4 T细胞、细胞毒性T细胞和B细胞进行染色。进行了特别关注免疫细胞位置的定量分析。
与对照组相比，CF患者的中性粒细胞、肥大细胞、树突状细胞、巨噬细胞、CD4 T细胞和细胞毒性T细胞显著增加，且CF患者气道周围的中性粒细胞增加不成比例。在CF肺部发现了大量淋巴滤泡，且它们的B细胞/T细胞组成不均衡。将CF患者分为男性和女性群体后，嗜酸性粒细胞、肥大细胞和CD4 T细胞在CF女性患者中特异性增加。在这个亚群体中，淋巴滤泡中的B细胞较少，CD8 T细胞较多。
这些数据表明CF肺部存在多样化的炎症反应，表现为髓样和淋巴样免疫细胞均增加。在我们的研究人群中，CF肺部炎症似乎具有性别特异性，因为嗜酸性粒细胞、肥大细胞和CD4 T细胞的显著增加在女性亚群体中尤为明显。
