Xie Zhiwei, Kim Jimin P, Cai Qing, Zhang Yi, Guo Jinshan, Dhami Ranjodh S, Li Li, Kong Bin, Su Yixue, Schug Kevin A, Yang Jian
Department of Biomedical Engineering, Materials Research Institutes, The Huck Institutes of Life Sciences, The Pennsylvania State University, University Park, PA 16801, United States.
Beijing Laboratory of Biomedical Materials, Beijing University of Chemical Technology, Beijing 100029, China.
Acta Biomater. 2017 Mar 1;50:361-369. doi: 10.1016/j.actbio.2017.01.019. Epub 2017 Jan 6.
Novel citric acid based photoluminescent dyes and biodegradable polymers are synthesized via a facile "one-pot" reaction. A comprehensive understanding of the fluorescence mechanisms of the resulting citric acid-based fluorophores is reported. Two distinct types of fluorophores are identified: a thiozolopyridine family with high quantum yield, long lifetime, and exceptional photostability, and a dioxopyridine family with relatively lower quantum yield, multiple lifetimes, and solvent-dependent band shifting behavior. Applications in molecular labeling and cell imaging were demonstrated. The above discoveries contribute to the field of fluorescence chemistry and have laid a solid foundation for further development of new fluorophores and materials that show promise in a diversity of fluorescence-based applications.
Photoluminescent materials are pivotal for fluorescence based imaging, labeling and sensing applications. Understanding their fluorescence mechanism is challenging and imperative. We develop a new class of citric acid-derived fluorescent materials in forms of polymers and small molecular dyes by a one-step solvent free reaction. We discovered two different classes of citric acid-derived fluorophores. A two-ring thiozolopyridine structure demonstrates strong fluorescence and exceptional resistance to photo-bleaching. A one-ring dioxopyridine exhibits relative weak fluorescence but with intriguing excitation and solvent-dependent emission wavelength shifting. Our methodology of synthesizing citric acid-derived fluorophores and the understanding on their luminescence are instrumental to the design and production of a large number of new photoluminescent materials for biological and biomedical applications.
通过简便的“一锅法”反应合成了新型柠檬酸基光致发光染料和可生物降解聚合物。报道了对所得柠檬酸基荧光团荧光机制的全面理解。鉴定出两种不同类型的荧光团：具有高量子产率、长寿命和出色光稳定性的噻唑并吡啶家族，以及具有相对较低量子产率、多个寿命和溶剂依赖性带移行为的二氧吡啶家族。展示了其在分子标记和细胞成像中的应用。上述发现为荧光化学领域做出了贡献，并为进一步开发在多种基于荧光的应用中显示出前景的新型荧光团和材料奠定了坚实基础。
光致发光材料对于基于荧光的成像、标记和传感应用至关重要。理解它们的荧光机制具有挑战性且势在必行。我们通过一步无溶剂反应开发了一类新型的柠檬酸衍生荧光材料，其形式为聚合物和小分子染料。我们发现了两类不同的柠檬酸衍生荧光团。双环噻唑并吡啶结构表现出强烈的荧光和出色的抗光漂白性。单环二氧吡啶表现出相对较弱的荧光，但具有有趣的激发和溶剂依赖性发射波长移动。我们合成柠檬酸衍生荧光团的方法及其对发光的理解有助于设计和生产大量用于生物和生物医学应用的新型光致发光材料。
