The State Key Laboratory of Tribology, Department of Mechanical Engineering, Tsinghua University, Beijing 100084, China.
Center for Photon Manufacturing Science and Technology, Jiangsu University, Zhenjiang 212013, China.
Talanta. 2015 Oct 1;143:107-113. doi: 10.1016/j.talanta.2015.04.015. Epub 2015 Apr 13.
In this work, high quantum yield and strong photoluminescent carbon quantum dots (C-QDs) are successfully synthesized via a facile and green hydrothermal method using citric acid and Tris as precursors. The as-synthesized C-QDs with a quantum yield (QY) as high as 52% were characterized by UV, FT-IR, TEM, XPS and fluorescence spectroscope. TEM results show that C-QDs are mono-dispersed spherical particles and the diameter distribution of C-QDs is 2.8±1.1 nm. The extraordinary photoluminescent properties and low cytotoxicity of C-QDs were obtained through optical property characterization and cytotoxicity assay. In addition, we found that the as-prepared C-QDs had a high affinity for Fe(3+) ions and the response toward Fe(3+) ions was highly linear (R(2)=0.997) over the concentration range from 2 to 50 μM, which could provide an effective platform for portable detection of Fe(3+) ions. Also, it is demonstrated that the photoluminescent C-QDs display hypotoxicity and are biocompatible for use as biosensors in living cells.
在这项工作中，通过一种简单而绿色的水热法，以柠檬酸和三羟甲基氨基甲烷为前驱体，成功合成了具有高光致发光量子产率和强荧光的碳量子点（C-QDs）。所合成的 C-QDs 的量子产率高达 52%，通过紫外可见吸收光谱、傅里叶变换红外光谱、透射电子显微镜、X 射线光电子能谱和荧光分光光度计对其进行了表征。TEM 结果表明，C-QDs 为单分散的球形颗粒，C-QDs 的直径分布为 2.8±1.1nm。通过光学性质表征和细胞毒性测定，获得了 C-QDs 的非凡光致发光性能和低细胞毒性。此外，我们发现所制备的 C-QDs 对 Fe(3+)离子具有高亲和力，并且对 Fe(3+)离子的响应在 2 至 50 μM 的浓度范围内具有高度线性（R(2)=0.997），这为便携式检测 Fe(3+)离子提供了一个有效的平台。此外，还证明了荧光 C-QDs 具有低细胞毒性，可用作活细胞中生物传感器的生物相容性材料。
