Vasilyeva Ekaterina, Abdulkhakov Sayar, Cherepnev Georgi, Martynova Ekaterina, Mayanskaya Irina, Valeeva Alina, Abdulkhakov Rustam, Safina Dilyara, Khaiboullina Svetlana, Rizvanov Albert
Volga Region Federal Medical Research Center of the Ministry of Public Health, Nizhny Novgorod, Russia.
Institute of Fundamental Medicine and Biology, Kazan Federal University, Kazan, Russia; Kazan Federal University Clinic, Kazan, Russia.
Mediators Inflamm. 2016;2016:7420127. doi: 10.1155/2016/7420127. Epub 2016 Dec 13.
Crohn's disease (CD) is a chronic inflammatory bowel disease that can be diagnosed at any age. There are two major patient groups based on diagnosis of this disease, before or after the age of 20 (juvenile/adolescent or adult), with disease progression in adults usually milder than in juvenile CD patients. Immune mechanisms have been suggested to play an important role in CD pathogenesis, with cytokines governing the development of the immune response. Upregulation of inflammatory cytokines in serum of juvenile and adult CD patients has been documented; still little is known about age-dependent differences in serum cytokine profiles of CD patients. We applied multiplex technology to analyze serum levels of 12 cytokines in juveniles and adults. We show that during the acute stage of the disease all CD patients have high serum levels of CXCL10, which remains upregulated during remission. Increased serum levels of TNF- and IL-6 during the acute stage was characteristic of juvenile CD patients, whereas adult CD patients had upregulated levels of GM-CSF and IFN-. Taken together, these results demonstrate age-dependent differences in cytokine profiles, which may affect the pathogenesis of CD in patients at different ages of disease onset.
克罗恩病（CD）是一种可在任何年龄被诊断出的慢性炎症性肠病。基于该病的诊断，有两个主要患者群体，即20岁之前（青少年期）或之后（成人期），成人期疾病进展通常比青少年期CD患者更轻。免疫机制被认为在CD发病机制中起重要作用，细胞因子调控免疫反应的发展。青少年和成人CD患者血清中炎性细胞因子上调已有记录；然而，关于CD患者血清细胞因子谱的年龄依赖性差异仍知之甚少。我们应用多重技术分析青少年和成人血清中12种细胞因子的水平。我们发现，在疾病急性期，所有CD患者血清CXCL10水平都很高，在缓解期仍保持上调。急性期血清TNF和IL-6水平升高是青少年CD患者的特征，而成人CD患者GM-CSF和IFN水平上调。综上所述，这些结果表明细胞因子谱存在年龄依赖性差异，这可能影响不同发病年龄患者的CD发病机制。
