Ma Yantian, Liu Fangpeng, Kong Zhaoyu, Yin Jianhua, Kou Wenbo, Wu Lan, Ge Gang
Key Laboratory of Poyang Lake Environment and Resource, Ministry of Education, School of Life Sciences, Nanchang University, Nanchang 330022, China.
Key Laboratory of Poyang Lake Environment and Resource, Ministry of Education, School of Life Sciences, Nanchang University, Nanchang 330022, China; Collaborative Innovation Center for Poyang Lake Basin Green Development and Water Security, Nanchang University, Nanchang 330031, China.
Archaea. 2016 Dec 13;2016:9278929. doi: 10.1155/2016/9278929. eCollection 2016.
Archaea plays an important role in the global geobiochemical circulation of various environments. However, much less is known about the ecological role of archaea in freshwater lake sediments. Thus, investigating the structure and diversity of archaea community is vital to understand the metabolic processes in freshwater lake ecosystems. In this study, sediment physicochemical properties were combined with the results from 16S rRNA clone library-sequencing to examine the sediment archaea diversity and the environmental factors driving the sediment archaea community structures. Seven sites were chosen from Poyang Lake, including two sites from the main lake body and five sites from the inflow river estuaries. Our results revealed high diverse archaea community in the sediment of Poyang Lake, including Bathyarchaeota (45.5%), Euryarchaeota (43.1%), Woesearchaeota (3.6%), Pacearchaeota (1.7%), Thaumarchaeota (1.4%), suspended Lokiarchaeota (0.7%), Aigarchaeota (0.2%), and Unclassified Archaea (3.8%). The archaea community compositions differed among sites, and sediment property had considerable influence on archaea community structures and distribution, especially total organic carbon (TOC) and metal lead (Pb) ( < 0.05). This study provides primary profile of sediment archaea distribution in freshwater lakes and helps to deepen our understanding of lake sediment microbes.
古菌在各种环境的全球地球生物化学循环中发挥着重要作用。然而，关于古菌在淡水湖沉积物中的生态作用，人们了解得还很少。因此，研究古菌群落的结构和多样性对于理解淡水湖生态系统中的代谢过程至关重要。在本研究中，将沉积物的理化性质与16S rRNA克隆文库测序结果相结合，以研究沉积物中古菌的多样性以及驱动沉积物古菌群落结构的环境因素。从鄱阳湖选取了7个位点，包括主湖体的2个位点和入湖河口的5个位点。我们的结果显示，鄱阳湖沉积物中古菌群落具有高度多样性，包括深古菌门（45.5%）、广古菌门（43.1%）、沃氏古菌门（3.6%）、帕氏古菌门（1.7%）、奇古菌门（1.4%）、悬浮洛基古菌门（0.7%）、艾古菌门（0.2%）和未分类古菌（3.8%）。不同位点的古菌群落组成不同，沉积物性质对古菌群落结构和分布有相当大的影响，尤其是总有机碳（TOC）和金属铅（Pb）（P<0.05）。本研究提供了淡水湖沉积物中古菌分布的初步概况，有助于加深我们对湖泊沉积物微生物的理解。
