Baricz Andreea, Cristea Adorján, Muntean Vasile, Teodosiu Gabriela, Andrei Adrian-Ştefan, Molnár Imola, Alexe Mircea, Rakosy-Tican Elena, Banciu Horia Leonard
Department of Molecular Biology and Biotechnology, Faculty of Biology and Geology, Babeş-Bolyai University, 5-7 Clinicilor Street, 400006, Cluj-Napoca, Romania.
Extremophiles. 2015 Mar;19(2):525-37. doi: 10.1007/s00792-015-0738-1. Epub 2015 Feb 14.
Perennially stratified salt lakes situated in the Transylvanian Basin (Central Romania) were surveyed for the diversity of culturable halophilic archaea (Fam. Halobacteriaceae). The physical and chemical characteristics of the waters indicated that all the investigated lakes were meromictic and neutral hypersaline. Samples collected from upper, intermediate, and deeper water layers and sediments were used for the isolation of halophilic strains followed by 16S rRNA gene-based identification and phenotypic characterization. The phylogenetic analysis of the 16S rRNA gene sequences revealed that all 191 isolates reported in this study and 43 strains previously isolated were affiliated with the family Halobacteriaceae and classified to 18 genera. Haloferax was the most frequently isolated genus (47 %), followed by Halobacterium spp. (12 %), and Halorubrum spp. (~11 %). Highest culturable diversity was detected in Brâncoveanu Lake, the oldest and saltiest of all studied lakes, while the opposite was observed in the most stable and least human-impacted Fără Fund Lake. One strain from Ursu Lake might possibly constitute a novel Halorubrum species as shown by phylogenetic analysis. Several haloarchaeal taxa recently described in Asian (i.e., Iran, China) saline systems were also identified as inhabiting the Transylvanian salt lakes thus expanding our knowledege on the geographic distribution of Halobacteriaceae.
对位于特兰西瓦尼亚盆地（罗马尼亚中部）的常年分层盐湖进行了调查，以研究可培养嗜盐古菌（嗜盐杆菌科）的多样性。水体的物理和化学特征表明，所有调查的湖泊都是半咸水湖且呈中性高盐。从上层、中层和深层水层以及沉积物中采集的样本用于分离嗜盐菌株，随后进行基于16S rRNA基因的鉴定和表型特征分析。对16S rRNA基因序列的系统发育分析表明，本研究中报道的所有191株分离株以及之前分离的43株菌株均隶属于嗜盐杆菌科，分为18个属。嗜盐富盐菌属是最常分离到的属（约47%），其次是嗜盐杆菌属（约12%）和嗜盐碱红菌属（约11%）。在所有研究湖泊中最古老且盐分最高的布兰科韦亚努湖中检测到最高的可培养多样性，而在最稳定且受人类影响最小的法勒芬德湖中则观察到相反的情况。系统发育分析表明，乌尔苏湖的一株菌株可能构成一个新的嗜盐碱红菌种。在亚洲（即伊朗、中国）盐渍系统中最近描述的几个嗜盐古菌类群也被鉴定为栖息在特兰西瓦尼亚盐湖中，从而扩展了我们对嗜盐杆菌科地理分布的认识。
