Naito Fernanda Y B, Melo Fernando L, Fonseca Maria Esther N, Santos Carlos A F, Chanes Carolina R, Ribeiro Bergmann M, Gilbertson Robert L, Boiteux Leonardo S, de Cássia Pereira-Carvalho Rita
University of Brasília, 70910-900, Brasília, Brazil.
Embrapa Vegetables, 70275-970, Brasília, Brazil.
Arch Virol. 2019 Jul;164(7):1907-1910. doi: 10.1007/s00705-019-04254-5. Epub 2019 Apr 10.
A new bipartite begomovirus (family Geminiviridae) was detected on cowpea (Vigna unguiculata) plants exhibiting bright golden mosaic symptoms on leaves under field conditions in Brazil. Complete consensus sequences of DNA-A and DNA-B components of an isolate of the virus (PE-088) were obtained by nanopore sequencing and confirmed by Sanger sequencing. The genome components presented the typical genomic organization of New World (NW) begomoviruses. Pairwise sequence comparisons revealed low levels of identity with other begomovirus species previously reported infecting cowpea around the world. Phylogenetic analysis using complete sequences of DNA-A components revealed that the closest relatives of PE-088 (85-87% nucleotide sequence identities) were three legume-infecting begomoviruses from Brazil: bean golden mosaic virus, macroptilium common mosaic virus and macroptilium yellow vein virus. According to the current classification criteria, PE-088 represents a new species in the genus Begomovirus, tentatively named as cowpea bright yellow mosaic virus (CoBYMV).
在巴西的田间条件下，在表现出叶片亮金色花叶症状的豇豆（Vigna unguiculata）植株上检测到一种新的双生病毒（双生病毒科）。通过纳米孔测序获得了该病毒一个分离株（PE - 088）的DNA - A和DNA - B组分的完整共有序列，并通过桑格测序进行了确认。基因组组分呈现出新大陆双生病毒的典型基因组结构。成对序列比较显示，与之前报道的世界各地感染豇豆的其他双生病毒种类的同一性水平较低。使用DNA - A组分的完整序列进行的系统发育分析表明，PE - 088的最亲近亲属（核苷酸序列同一性为85 - 87%）是来自巴西的三种感染豆科植物的双生病毒：菜豆金色花叶病毒、大翼豆普通花叶病毒和大翼豆黄脉病毒。根据目前的分类标准，PE - 088代表双生病毒属中的一个新物种，暂命名为豇豆亮黄花叶病毒（CoBYMV）。
