Tinkov Alexey A, Popova Elizaveta V, Gatiatulina Evgenia R, Skalnaya Anastasia A, Yakovenko Elena N, Alchinova Irina B, Karganov Mikhail Y, Skalny Anatoly V, Nikonorov Alexandr A
Laboratory of Biotechnology and Applied Bioelementology, Yaroslavl State University, Russia.
All-Russian Research Institute of Medicinal and Aromatic Plants (VILAR), Moscow, Russia.
Acta Sci Pol Technol Aliment. 2016 Jan-Mar;15(1):99-105. doi: 10.17306/J.AFS.2016.1.10.
Limited data on adipose tissue zinc content in obesity exist. At the same time, the association between adipose tissue zinc content and metabolic parameters in dietary-induced obesity is poorly studied. Therefore, the primary objective of this study is to assess adipose tissue zinc content and its association with morphometric parameters, adipokine spectrum, proinflammatory cytokines, and apolipoprotein profile in high fat fed Wistar rats.
A total of 48 adult female Wistar rats were used in the present study. Rats were fed either control (10% of fat) or high fat diet (31.6% of fat). Adipose tissue zinc content was assessed using inductively coupled plasma mass spectrometry. Rats' serum was examined for adiponectin, leptin, insulin, interleukin-6, and tumor necrosis factor-α using enzyme-linked immunosorbent assay kits. Serum glucose and apolipoprotein spectrum were also evaluated.
High fat feeding resulted in a significant 34% decrease in adipose tissue zinc content in comparison to the control values. Fat pad zinc levels were significantly inversely associated with morphometric parameters, circulating leptin, insulin, tumor necrosis factor-α levels and HOMA-IR values. At the same time, a significant correlation with apolipoprotein A1 concentration was observed.
Generally, the obtained data indicate that (1) high fat feeding results in decreased adipose tissue zinc content; (2) adipose tissue zinc content is tightly associated with excessive adiposity, inflammation, insulin resistance and potentially atherogenic changes.
关于肥胖症患者脂肪组织锌含量的数据有限。与此同时，饮食诱导肥胖中脂肪组织锌含量与代谢参数之间的关联研究较少。因此，本研究的主要目的是评估高脂喂养的Wistar大鼠脂肪组织锌含量及其与形态学参数、脂肪因子谱、促炎细胞因子和载脂蛋白谱的关联。
本研究共使用48只成年雌性Wistar大鼠。大鼠分别喂食对照饮食（脂肪含量10%）或高脂饮食（脂肪含量31.6%）。使用电感耦合等离子体质谱法评估脂肪组织锌含量。使用酶联免疫吸附测定试剂盒检测大鼠血清中的脂联素、瘦素、胰岛素、白细胞介素-6和肿瘤坏死因子-α。还评估了血清葡萄糖和载脂蛋白谱。
与对照值相比，高脂喂养导致脂肪组织锌含量显著降低34%。脂肪垫锌水平与形态学参数、循环瘦素、胰岛素、肿瘤坏死因子-α水平和HOMA-IR值显著负相关。同时，观察到与载脂蛋白A1浓度存在显著相关性。
总体而言，获得的数据表明：（1）高脂喂养导致脂肪组织锌含量降低；（2）脂肪组织锌含量与过度肥胖、炎症、胰岛素抵抗以及潜在的动脉粥样硬化变化密切相关。
