Santander Javier, Vasquez Jose I, Segovia Cristopher, Santos Leonardo, Turra Gabriel, Huber Karen, Robeson James
Department of Ocean Sciences, Memorial University of Newfoundland, St. John's, Canada ; Universidad Mayor, Faculty of Sciences, Huechuraba, Chile ; Memorial University of Newfoundland, Faculty of Sciences, Ocean Science Centre, Marine Microbial Pathogenesis and Vaccinology Laboratory AX3005, 0 Marine Lab Rd, Logy Bay, NL A1K 3E6 Canada.
Universidad Mayor, Faculty of Sciences, Huechuraba, Chile.
Stand Genomic Sci. 2017 Jan 5;12:1. doi: 10.1186/s40793-016-0218-y. eCollection 2017.
serovar Enteritidis is one of the most common causes of Salmonellosis worldwide. Utilization of bacteriophages as prophylactic agents is a practical solution to prevent Salmonellosis in ready-to-eat products. Shelf stability is one of the desirable properties for prophylactic bacteriophages. Here, we describe the phenotype, genome, and phylogeny of SE1C and SE4S bacteriophages. SE1C and SE4S were previously isolated from pickle sauce and ground beef respectively and selected for their significant shelf stability. SE1C and SE4S showed a broad serovar range, infecting several serovars. The viral particles showed an icosahedral head structure and flexible tail, a typical morphology of the family. SE1C and SE4C genomes consists of dsDNA of 41,720 bp and 41,768 bp with 49.73% and 49.78% G + C, respectively. Comparative genomic analysis reveals a mosaic relationship between serovar Enteritidis phages isolated from Valparaiso, Chile.
肠炎血清型是全球范围内沙门氏菌病最常见的病因之一。利用噬菌体作为预防剂是预防即食产品中沙门氏菌病的一种切实可行的解决方案。货架稳定性是预防性噬菌体所需的特性之一。在此，我们描述了SE1C和SE4S噬菌体的表型、基因组和系统发育。SE1C和SE4S先前分别从泡菜酱和碎牛肉中分离出来，并因其显著的货架稳定性而被选中。SE1C和SE4S表现出广泛的血清型范围，可感染多种血清型。病毒颗粒呈现二十面体头部结构和灵活的尾部，这是该家族的典型形态。SE1C和SE4C基因组分别由41,720 bp和41,768 bp的双链DNA组成，G + C含量分别为49.73%和49.78%。比较基因组分析揭示了从智利瓦尔帕莱索分离的肠炎血清型噬菌体之间的镶嵌关系。
