Nakahira Rei, Michishita Masaki, Kato Misaki, Okuno Yuki, Hatakeyama Hitoshi, Yoshimura Hisashi, Azakami Daigo, Ochiai Kazuhiko, Bonkobara Makoto, Takahashi Kimimasa
Departments of Veterinary Pathology (Nakahira, Michishita, Kato, Okuno, Takahashi), School of Veterinary Medicine, Nippon Veterinary and Life Science University, Tokyo, Japan.
Comparative Cellular Biology (Hatakeyama), School of Veterinary Medicine, Nippon Veterinary and Life Science University, Tokyo, Japan.
J Vet Diagn Invest. 2017 Jan;29(1):105-108. doi: 10.1177/1040638716673126. Epub 2016 Dec 20.
A 3-y-old male miniature Dachshund was presented with an ~0.8 cm diameter mass in the right mandibular region. Fourteen months later, the mass was 5 × 4 × 3 cm. Grossly, the mass was encapsulated and was homogeneously gray-white on cut surface. Microscopically, the mass was composed of large, round to polygonal tumor cells that were arranged in solid nests and cords separated by a fibrovascular stroma. Tumor cells had large, round, hypochromatic nuclei containing large prominent nucleoli and abundant eosinophilic cytoplasm containing dark blue granules visible with phosphotungstic acid-hematoxylin stain. Metastasis was observed in the mandibular lymph node. Immunohistochemically, tumor cells were positive for CK AE1/AE3, low-molecular-weight CK (CAM5.2), E-cadherin, mitochondria ATPase beta subunit, and S100, but were negative for vimentin, carcinoembryonic antigen, p63, CK14, CD10, and chromogranin A. Ultrastructurally, tumor cells contained numerous mitochondria. Therefore, the tumor was diagnosed as an oncocytic carcinoma of the mandibular gland.
一只3岁雄性迷你腊肠犬右侧下颌区域出现一个直径约0.8 cm的肿块。14个月后，肿块大小为5×4×3 cm。大体上，肿块有包膜，切面呈均匀的灰白色。显微镜下，肿块由大的圆形至多边形肿瘤细胞组成，这些细胞排列成实性巢状和条索状，被纤维血管间质分隔。肿瘤细胞有大的圆形、淡染核，含有大而突出的核仁，以及丰富的嗜酸性细胞质，用磷钨酸苏木精染色可见深蓝色颗粒。在下颌淋巴结中观察到转移。免疫组化显示，肿瘤细胞对细胞角蛋白AE1/AE3、低分子量细胞角蛋白（CAM5.2）、E-钙黏蛋白、线粒体ATP酶β亚基和S100呈阳性，但对波形蛋白、癌胚抗原、p63、细胞角蛋白14、CD10和嗜铬粒蛋白A呈阴性。超微结构上，肿瘤细胞含有大量线粒体。因此，该肿瘤被诊断为下颌腺嗜酸性癌。
